Structure of yeast poly(A) polymerase in complex with a peptide from Fip1, an intrinsically disordered protein.

@article{Meinke2008StructureOY,
  title={Structure of yeast poly(A) polymerase in complex with a peptide from Fip1, an intrinsically disordered protein.},
  author={Gretchen Meinke and Chukwudi Ezeokonkwo and Paul Balbo and Walter F. Stafford and Claire Moore and Andrew R Bohm},
  journal={Biochemistry},
  year={2008},
  volume={47 26},
  pages={
          6859-69
        }
}
In yeast, the mRNA processing enzyme poly(A) polymerase is tethered to the much larger 3'-end processing complex via Fip1, a 36 kDa protein of unknown structure. We report the 2.6 A crystal structure of yeast poly(A) polymerase in complex with a peptide containing residues 80-105 of Fip1. The Fip1 peptide binds to the outside surface of the C-terminal domain of the polymerase. On the basis of this structure, we designed a mutant of the polymerase (V498Y, C485R) that is lethal to yeast. The… CONTINUE READING

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