Structure of yeast Argonaute with guide RNA

@inproceedings{Nakanishi2012StructureOY,
  title={Structure of yeast Argonaute with guide RNA},
  author={Kotaro Nakanishi and David E Weinberg and David P. Bartel and Dinshaw J. Patel},
  booktitle={Nature},
  year={2012}
}
The RNA-induced silencing complex, comprising Argonaute and guide RNA, mediates RNA interference. Here we report the 3.2 Å crystal structure of Kluyveromyces polysporus Argonaute (KpAGO) fortuitously complexed with guide RNA originating from small-RNA duplexes autonomously loaded and processed by recombinant KpAGO. Despite their diverse sequences, guide-RNA nucleotides 1–8 are positioned similarly, with sequence-independent contacts to bases, phosphates and 2′-hydroxyl groups pre-organizing the… CONTINUE READING
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