Structure of the native Sec61 protein-conducting channel

@inproceedings{Pfeffer2015StructureOT,
  title={Structure of the native Sec61 protein-conducting channel},
  author={Stefan Pfeffer and Laura Burbaum and Pia Unverdorben and Markus Pech and Yuxiang Chen and Richard Zimmermann and Roland Beckmann and Friedrich F{\"o}rster},
  booktitle={Nature communications},
  year={2015}
}
In mammalian cells, secretory and membrane proteins are translocated across or inserted into the endoplasmic reticulum (ER) membrane by the universally conserved protein-conducting channel Sec61, which has been structurally studied in isolated, detergent-solubilized states. Here we structurally and functionally characterize native, non-solubilized ribosome-Sec61 complexes on rough ER vesicles using cryo-electron tomography and ribosome profiling. Surprisingly, the 9-Å resolution subtomogram… CONTINUE READING
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References

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SHOWING 1-10 OF 37 REFERENCES

Mechanisms of integral membrane protein insertion and folding

  • F. Cymer, G. von Heijne, S. H. White
  • J. Mol. Biol
  • 2015

Structure of the SecY channel during initiation of protein translocation

  • E Park
  • Nature 506,
  • 2014

Structure of the immature HIV-1 capsid in intact virus particles at 8.8 A resolution

  • Schur, K F.
  • Nature 517,
  • 2014

Structure of the mammalian oligosaccharyl-transferase complex in the native ER protein translocon

  • S Pfeffer
  • Nat. Commun
  • 2014

Structures of the Sec61 complex engaged in nascent peptide translocation or membrane insertion

  • M Gogala
  • Nature 506,
  • 2014

Determination of protein structure at 8.5 A resolution using cryo-electron tomography and subtomogram averaging

  • F. K. Schur, W. J. Hagen, A. de Marco, J. A. Briggs
  • J. Struct. Biol
  • 2013

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