Structure of the manganese complex of photosystem II upon removal of the 33-kilodalton extrinsic protein: an X-ray absorption spectroscopy study.

@article{Cole1987StructureOT,
  title={Structure of the manganese complex of photosystem II upon removal of the 33-kilodalton extrinsic protein: an X-ray absorption spectroscopy study.},
  author={James L. Cole and Vittal K. Yachandra and Ann E McDermott and R. D. Guiles and R David Britt and S L Dexheimer and Kenneth Sauer and Melvin Klein},
  journal={Biochemistry},
  year={1987},
  volume={26 19},
  pages={5967-73}
}
The structure of the Mn complex of photosystem II (PSII) was studied by X-ray absorption spectroscopy. Oxygen-evolving spinach PSII membranes containing 4-5 Mn/PSII were treated with 0.8 M CaCl2 to extract the 33-, 24-, and 16-kilodalton (kDa) extrinsic membrane proteins. Mn was not released by this treatment, but subsequent incubation at low Cl- concentration generated preparations containing 2 Mn/PSII. The Mn X-ray absorption K-edge spectrum of the CaCl2-washed preparation containing 4 Mn… CONTINUE READING