Structure of the human immune interferon gene

  title={Structure of the human immune interferon gene},
  author={Patrick W. Gray and David Norman Van Goeddel},
Sequence determination of cloned cDNAs and genes of the three classes of interferon (IFN-α, –β and –γ) has revealed more than a dozen members of the human IFN-α gene family1–7 and a single gene for IFN-β4,8–14. These genes are found on chromosome 9 (ref. 15) and contain no introns1,5–7,11–14. We recently reported16 that the 146-amino acid sequence of mature IFN-γ deduced from the nucleotide sequence of a cloned cDNA was quite unrelated to those of the other IFNs, and that the gene for IFN… 

The presence of homologous regions between interferon sequences

Comparison of human and murine α-and β-type interferons suggests the presence of two major homology blocks within type I interferon sequences.

Efficient expression in Escherichia coli of two species of human interferon-alpha and their hybrid molecules.

An expression plasmid which directs the synthesis of the mature IFN-alpha I' peptide was constructed using vectors carrying the lpp/lac promoter and "runaway" replicon and the specific antiviral activities of the hybrid IFNs on human and bovine cells were similar to that of the amino-terminal parental IFN peptide.

Coordinately Expressed and Encode Functional Polypeptides

It is demonstrated that the class I and class II IFN-a genes are coordinately induced in response to viral infection.

Structure of the human interleukin 2 gene.

Two species of EcoRI-cleaved DNA segments are cloned that together cover the entire sequence for the human interleukin 2 gene and the nucleotide sequence of the gene and its flanking regions are determined.

Polymorphisms of the human IFNG gene noncoding regions

An invariant proximal human IFNG promoter is reported and the existence of multiple intronic variants and a potentially functional 3′UTR polymorphism is reported.

The interferon genes.




At least three human type alpha interferons: structure of alpha 2.

The sequence of a human leukocyte-derived complementary DNA, Hif-2h, which directs the formation in Escherichia coli of a polypeptide, IFN-alpha 1, with interferon (IFN) activity has been described, revealing the presence of at least eightIFN-related genes.

The structure of one of the eight or more distinct chromosomal genes for human interferon-α

The 12 Interferon (IFN)-related sequences detected in a human gene bank fall into not less than eight distinct classes, indicating that there are at least eight IFN-related genes. Most, if not all,

DNA sequence of a major human leukocyte interferon gene.

A comparison of seven human IFN-alpha sequences shows that they are homologous in the 5' flanking region and contain identical "TATA box" sequences.

Structure of a chromosomal gene for human interferon beta.

  • S. OhnoT. Taniguchi
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
From the sequence data and the result of nuclease S1 mapping experiments, it is concluded that the interferon beta(1) gene is devoid of intervening sequences.

DNA sequences necessary for transcription of the rabbit β-globin gene in vivo

Comparison of the level of β-globin transcripts in a variety of deletion mutants shows that for efficient transcription, both the ATA or Goldberg–Hogness box, and a region between 100 and 58 base pairs in front of the site at which transcription is initiated, are required.

Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.

It is argued that the T-A-T-A -A-A and A-G-A motif is a specificity element, a selector of eukaryotic gene transcription, and that deletion of H2A gene-specific conserved DNA sequences upstream from this motif enhanced mRNA synthesis.