Structure of the human immune interferon gene

@article{Gray1982StructureOT,
  title={Structure of the human immune interferon gene},
  author={Patrick W. Gray and David Norman Van Goeddel},
  journal={Nature},
  year={1982},
  volume={298},
  pages={859-863}
}
Sequence determination of cloned cDNAs and genes of the three classes of interferon (IFN-α, –β and –γ) has revealed more than a dozen members of the human IFN-α gene family1–7 and a single gene for IFN-β4,8–14. These genes are found on chromosome 9 (ref. 15) and contain no introns1,5–7,11–14. We recently reported16 that the 146-amino acid sequence of mature IFN-γ deduced from the nucleotide sequence of a cloned cDNA was quite unrelated to those of the other IFNs, and that the gene for IFN… 

The presence of homologous regions between interferon sequences

Comparison of human and murine α-and β-type interferons suggests the presence of two major homology blocks within type I interferon sequences.

Efficient expression in Escherichia coli of two species of human interferon-alpha and their hybrid molecules.

An expression plasmid which directs the synthesis of the mature IFN-alpha I' peptide was constructed using vectors carrying the lpp/lac promoter and "runaway" replicon and the specific antiviral activities of the hybrid IFNs on human and bovine cells were similar to that of the amino-terminal parental IFN peptide.

Coordinately Expressed and Encode Functional Polypeptides

It is demonstrated that the class I and class II IFN-a genes are coordinately induced in response to viral infection.

Structure of the human interleukin 2 gene.

Two species of EcoRI-cleaved DNA segments are cloned that together cover the entire sequence for the human interleukin 2 gene and the nucleotide sequence of the gene and its flanking regions are determined.

Polymorphisms of the human IFNG gene noncoding regions

An invariant proximal human IFNG promoter is reported and the existence of multiple intronic variants and a potentially functional 3′UTR polymorphism is reported.

The interferon genes.

...

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    Proceedings of the National Academy of Sciences of the United States of America
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