Structure of the human immune interferon gene

@article{Gray1982StructureOT,
  title={Structure of the human immune interferon gene},
  author={Patrick W. Gray and David Norman Van Goeddel},
  journal={Nature},
  year={1982},
  volume={298},
  pages={859-863}
}
Sequence determination of cloned cDNAs and genes of the three classes of interferon (IFN-α, –β and –γ) has revealed more than a dozen members of the human IFN-α gene family1–7 and a single gene for IFN-β4,8–14. These genes are found on chromosome 9 (ref. 15) and contain no introns1,5–7,11–14. We recently reported16 that the 146-amino acid sequence of mature IFN-γ deduced from the nucleotide sequence of a cloned cDNA was quite unrelated to those of the other IFNs, and that the gene for IFN… Expand
Structure of the chicken interferon-γ gene, and comparison to mammalian homologues
TLDR
The sequence of the chicken interferon-γ (ifn-γ) gene was determined, one of the first non-mammalian cytokine gene structures to be elucidated, with no polymorphisms yet identified using either RFLP or SSCP in the coding region. Expand
The presence of homologous regions between interferon sequences
TLDR
Comparison of human and murine α-and β-type interferons suggests the presence of two major homology blocks within type I interferon sequences. Expand
Structure and characterization of a murine chromosomal fragment containing the interferon beta gene.
TLDR
Comparison of the MuIFN beta 5' flanking region with those from other species reveals similarities in the sequences required for the regulated expression of such inducible genes. Expand
Efficient expression in Escherichia coli of two species of human interferon-alpha and their hybrid molecules.
TLDR
An expression plasmid which directs the synthesis of the mature IFN-alpha I' peptide was constructed using vectors carrying the lpp/lac promoter and "runaway" replicon and the specific antiviral activities of the hybrid IFNs on human and bovine cells were similar to that of the amino-terminal parental IFN peptide. Expand
Structural relationship of human interferon alpha genes and pseudogenes.
TLDR
The finding that at least two IFN-alpha genes appear to be natural hybrids of other IFN -alpha genes, and that two distinct IFn-alpha loci have completely identical coding sequences, although their flanking regions are different, is evidence for information exchange between the individual genes. Expand
Structure of the human interleukin 2 gene.
TLDR
Two species of EcoRI-cleaved DNA segments are cloned that together cover the entire sequence for the human interleukin 2 gene and the nucleotide sequence of the gene and its flanking regions are determined. Expand
Expression of Human Interferon-γ in Heterologous Systems Genentech, Inc. Contribution no. 134.
TLDR
This chapter discusses the expression of human interferon-γ (IFN-γ) in heterologous systems, which is distinct from the leukocyte interferons (IFNs-α) or fibroblast interfer on the basis of neutralization studies with antibodies raised against different species. Expand
Polymorphisms of the human IFNG gene noncoding regions
TLDR
An invariant proximal human IFNG promoter is reported and the existence of multiple intronic variants and a potentially functional 3′UTR polymorphism is reported. Expand
The interferon genes.
TLDR
This chapter discusses the types, effects, and properties of interferons (IFN), which are induced by viral infection or the treatment of a variety of cells with poly(I)·poly(C) and are usually acid-stable. Expand
Two distinct families of human and bovine interferon-alpha genes are coordinately expressed and encode functional polypeptides
The classical human interferon-alpha (HuIFN-alpha) gene family is estimated to consist of 15 or more nonallelic members which encode proteins sharing greater than 77% amino acid sequence homology.Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 38 REFERENCES
At least three human type alpha interferons: structure of alpha 2.
TLDR
The sequence of a human leukocyte-derived complementary DNA, Hif-2h, which directs the formation in Escherichia coli of a polypeptide, IFN-alpha 1, with interferon (IFN) activity has been described, revealing the presence of at least eightIFN-related genes. Expand
The structure of one of the eight or more distinct chromosomal genes for human interferon-α
The 12 Interferon (IFN)-related sequences detected in a human gene bank fall into not less than eight distinct classes, indicating that there are at least eight IFN-related genes. Most, if not all,Expand
DNA sequence of a major human leukocyte interferon gene.
TLDR
A comparison of seven human IFN-alpha sequences shows that they are homologous in the 5' flanking region and contain identical "TATA box" sequences. Expand
Structure of a chromosomal gene for human interferon beta.
  • S. Ohno, T. Taniguchi
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1981
TLDR
From the sequence data and the result of nuclease S1 mapping experiments, it is concluded that the interferon beta(1) gene is devoid of intervening sequences. Expand
The isolation and characterization of linked δ- and β-globin genes from a cloned library of human DNA
TLDR
The two independently isolated β-globin clones differ from each other by the presence of a Pst I restriction enzyme cleavage site within the large intervening sequence of the δ- globin gene of one of the clones, which suggests that the human DNA carried in the two clones was derived from two homologous chromosomes which were heterozygous for the PstI restriction enzyme recognition sequence. Expand
Nucleotide sequence of the chromosomal gene for human fibroblast (beta 1) interferon and of the flanking regions.
The nucleotide sequence of the human fibroblast (beta 1) interferon chromosomal gene and its flanking regions was determined. These results confirm the absence of intervening sequences in the gene.Expand
The absence of introns within a human fibroblast interferon gene.
TLDR
Having isolated a chromosomal human fibroblast interferon gene from a gene bank, it is concluded from nucleotide sequencing studies that there is a complete absence of introns within the RNA-coding region. Expand
DNA sequences necessary for transcription of the rabbit β-globin gene in vivo
TLDR
Comparison of the level of β-globin transcripts in a variety of deletion mutants shows that for efficient transcription, both the ATA or Goldberg–Hogness box, and a region between 100 and 58 base pairs in front of the site at which transcription is initiated, are required. Expand
Expression of human immune interferon cDNA in E. coli and monkey cells
TLDR
The polypeptide produced through expression of this DNA sequence in Escherichia coli or cultured monkey cells had properties characteristic of authentic human IFN-γ. Expand
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.
  • R. Grosschedl, M. Birnstiel
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1980
TLDR
It is argued that the T-A-T-A -A-A and A-G-A motif is a specificity element, a selector of eukaryotic gene transcription, and that deletion of H2A gene-specific conserved DNA sequences upstream from this motif enhanced mRNA synthesis. Expand
...
1
2
3
4
...