Structure of the human activated spliceosome in three conformational states

@article{Zhang2018StructureOT,
  title={Structure of the human activated spliceosome in three conformational states},
  author={Xiaofeng Zhang and Chuangye Yan and Xiechao Zhan and Lijia Li and Jianlin Lei and Yigong Shi},
  journal={Cell Research},
  year={2018},
  volume={28},
  pages={307 - 322}
}
During each cycle of pre-mRNA splicing, the pre-catalytic spliceosome (B complex) is converted into the activated spliceosome (Bact complex), which has a well-formed active site but cannot proceed to the branching reaction. Here, we present the cryo-EM structure of the human Bact complex in three distinct conformational states. The EM map allows atomic modeling of nearly all protein components of the U2 small nuclear ribonucleoprotein (snRNP), including three of the SF3a complex and seven of… Expand
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