Structure of the glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase.

@article{Redman1994StructureOT,
  title={Structure of the glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase.},
  author={Christopher A. Redman and Jane E. Thomas-Oates and Satoshi Ogata and Yukio Ikehara and Michael A J Ferguson},
  journal={The Biochemical journal},
  year={1994},
  volume={302 ( Pt 3)},
  pages={861-5}
}
The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting glycosylphosphatidylinositol-peptide was subjected to compositional analysis and chemical and enzymic modifications. The neutral-glycan fraction, prepared by dephosphorylation followed by HNO2 deamination and reduction, was sequenced using exoglycosidases and acetolysis. The phosphatidylinositol moiety… CONTINUE READING