Structure of the A Site of Escherichia coli 16S Ribosomal RNA Complexed with an Aminoglycoside Antibiotic

@article{Fourmy1996StructureOT,
  title={Structure of the A Site of Escherichia coli 16S Ribosomal RNA Complexed with an Aminoglycoside Antibiotic},
  author={Dominique Fourmy and Michael I. Recht and Scott C. Blanchard and Joseph D. Puglisi},
  journal={Science},
  year={1996},
  volume={274},
  pages={1367 - 1371}
}
Aminoglycoside antibiotics that bind to 30S ribosomal A-site RNA cause misreading of the genetic code and inhibit translocation. The aminoglycoside antibiotic paromomycin binds specifically to an RNA oligonucleotide that contains the 30S subunit A site, and the solution structure of the RNA-paromomycin complex was determined by nuclear magnetic resonance spectroscopy. The antibiotic binds in the major groove of the model A-site RNA within a pocket created by an A-A base pair and a single bulged… 
Structural origins of gentamicin antibiotic action
TLDR
The structure of gentamicin leads to a general model for specific ribosome recognition by aminoglycoside antibiotics and a possible mechanism for translational inhibition and miscoding, and provides a structural rationale for chemical synthesis of novel am inoglycosides.
Structural origins of aminoglycoside specificity for prokaryotic ribosomes.
TLDR
It is suggested that eukaryotic ribosomal RNA has a shallow binding pocket for aminoglycosides, which accommodates only certain antibiotics.
Binding of neomycin-class aminoglycoside antibiotics to the A-site of 16 S rRNA.
TLDR
NMR data show that rings I and II of neomycin-class aminoglycosides are sufficient to confer specificity to the binding of the antibiotics to the model A-site RNA.
Paromomycin binding induces a local conformational change in the A-site of 16 S rRNA.
TLDR
Two universally conserved residues of the A site of 16 S rRNA, A1492 and A1493, are displaced towards the minor groove of the RNA helix in presence of antibiotic and changes in the RNA conformation suggest a mechanism of action of aminoglycosides on translation.
Basis for prokaryotic specificity of action of aminoglycoside antibiotics
TLDR
The results indicate that the identity of the nucleotide at position 1408 is a major determinant of specificity of aminoglycoside action, and agree with prior structural studies of am inoglycosides–rRNA complexes.
Recognition of Cognate Transfer RNA by the 30S Ribosomal Subunit
Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved
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