Structure of the 70S Ribosome Complexed with mRNA and tRNA

@article{Selmer2006StructureOT,
  title={Structure of the 70S Ribosome Complexed with mRNA and tRNA},
  author={Maria Selmer and Christine M. Dunham and Frank V. Murphy and Albert Weixlbaumer and Sabine Petry and Ann C. Kelley and John R. Weir and Venki Ramakrishnan},
  journal={Science},
  year={2006},
  volume={313},
  pages={1935 - 1942}
}
The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those… 

Interactions of the ribosome with mRNA and tRNA.

Structures of the Bacterial Ribosome at 3.5 Å Resolution

Swiveling of the head of the small subunit observed in the present structures, coupled to the ratchet-like motion of the two subunits observed previously, suggests a mechanism for the final movements of messenger RNA and transfer RNAs during translocation.

Crystal structure of the ribosome recycling factor bound to the ribosome

The crystal structure of the Thermus thermophilus RRF bound to a 70S ribosomal complex containing a stop codon in the A site, a transfer RNA anticodon stem-loop in the P site and tRNAfMet in the E site is described, demonstrating that structures of translation factors bound to 70S Ribosomes can be determined at reasonably high resolution.

Interaction of tRNA with Eukaryotic Ribosome

Data obtained by means of cryo-electron microscopy and X-ray crystallography applied to various model ribosomal complexes, site-directed cross-linking with the use of tRNA derivatives bearing chemically or photochemically reactive groups in the CCA-terminal fragment and chemical probing of 28S rRNA in the region of the peptidyl transferase center are reviewed.

Structural basis for translation termination on the 70S ribosome

The crystal structure of the Thermus thermophilus 70S ribosome in complex with the release factor RF1, tRNA and a messenger RNA containing a UAA stop codon is described, at 3.2 Å resolution.

Intersubunit Bridges of the Bacterial Ribosome.

Insights into Translational Termination from the Structure of RF2 Bound to the Ribosome

The crystal structure of RF2 in complex with its cognate UGA stop codon in the 70S ribosome provides insight into how RF2 specifically recognizes the stopcodon; it also suggests a model for the role of a universally conserved GGQ motif in the catalysis of peptide release.

A structural understanding of the dynamic ribosome machine

  • T. Steitz
  • Biology, Chemistry
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  • 2008
The structure of its larger subunit revealed that the ribosome is a ribozyme with RNA at the heart of its enzymatic activity that catalyses peptide bond formation.

The crystal structure of unmodified tRNAPhe from Escherichia coli

It is shown that in the absence of modifications the overall fold of the tRNA is essentially the same as that of mature tRNA, but there are a number of significant structural differences, such as rearrangements in a triplet base pair and a widened angle between the acceptor and anticodon stems.

Structures of the Bacterial Ribosome in Classical and Hybrid States of tRNA Binding

Two crystal structures indicate how conformational changes in the ribosome assist protein synthesis and help to explain how the ratchet-like motion of the two ribosomal subunits contributes to the mechanisms of translocation, termination, and Ribosome recycling.
...

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