Structure of plectonemically supercoiled DNA.

  title={Structure of plectonemically supercoiled DNA.},
  author={T. Christian Boles and James Garrett White and Nicholas R. Cozzarelli},
  journal={Journal of molecular biology},
  volume={213 4},
Using electron microscopy and topological methods, we have deduced an average structure for negatively supercoiled circular DNA in solution. Our data suggest that DNA has a branched plectonemic (interwound) form over the range of supercoiling tested. The length of the superhelix axis is constant at 41% of the DNA length, whereas the superhelix radius decreases essentially hyperbolically as supercoiling increases. The number of supercoils is 89% of the linking deficit. Both writhe and twist… CONTINUE READING

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