Structure of collagen receptor integrin α(1)I domain carrying the activating mutation E317A.

@article{Lahti2011StructureOC,
  title={Structure of collagen receptor integrin α(1)I domain carrying the activating mutation E317A.},
  author={Matti Lahti and Eva Bligt and Henri Niskanen and Vimal Parkash and Anna-Maria Brandt and Johanna Jokinen and Pekka Patrikainen and Jarmo K{\"a}pyl{\"a} and Jyrki Heino and Tiina A Salminen},
  journal={The Journal of biological chemistry},
  year={2011},
  volume={286 50},
  pages={43343-51}
}
We have analyzed the structure and function of the integrin α(1)I domain harboring a gain-of-function mutation E317A. To promote protein crystallization, a double variant with an additional C139S mutation was used. In cell adhesion assays, the E317A mutation promoted binding to collagen. Similarly, the double mutation C139S/E317A increased adhesion compared with C139S alone. Furthermore, soluble α(1)I C139S/E317A was a higher avidity collagen binder than α(1)I C139S, indicating that the double… CONTINUE READING

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