Structure of an RNA polymerase II preinitiation complex.

@article{Murakami2015StructureOA,
  title={Structure of an RNA polymerase II preinitiation complex.},
  author={Kenji Murakami and Kuang-Lei Tsai and Nir Kalisman and David A. Bushnell and Francisco J. Asturias and Roger D. Kornberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2015},
  volume={112 44},
  pages={13543-8}
}
The structure of a 33-protein, 1.5-MDa RNA polymerase II preinitiation complex (PIC) was determined by cryo-EM and image processing at a resolution of 6-11 Å. Atomic structures of over 50% of the mass were fitted into the electron density map in a manner consistent with protein-protein cross-links previously identified by mass spectrometry. The resulting… CONTINUE READING