Structure of an Aprataxin–DNA complex with insights into AOA1 Neurodegenerative Disease

@inproceedings{Tumbale2011StructureOA,
  title={Structure of an Aprataxin–DNA complex with insights into AOA1 Neurodegenerative Disease},
  author={Percy P Tumbale and C. Denise Appel and Rolf Kraehenbuehl and Patrick D. Robertson and Jessica S. Williams and Joe M Krahn and Ivan Ahel and R Scott Williams},
  booktitle={Nature Structural &Molecular Biology},
  year={2011}
}
DNA ligases finalize DNA replication and repair through DNA nick-sealing reactions that can abort to generate cytotoxic 5′-adenylation DNA damage. Aprataxin (Aptx) catalyzes direct reversal of 5′-adenylate adducts to protect genome integrity. Here the structure of a Schizosaccharomyces pombe Aptx–DNA–AMP–Zn2+ complex reveals active site and DNA interaction clefts formed by fusing a histidine triad (HIT) nucleotide hydrolase with a DNA minor groove–binding C2HE zinc finger (Znf). An Aptx helical… CONTINUE READING