Structure of a ribulose 5‐phosphate 3‐epimerase from Plasmodium falciparum

  title={Structure of a ribulose 5‐phosphate 3‐epimerase from Plasmodium falciparum},
  author={Jonathan M. Caruthers and J{\"u}rgen Bosch and Frederick S. Buckner and Wesley C. Van Voorhis and Peter J. Myler and Elizabeth A. Worthey and Christopher Mehlin and Erica Boni and George T. DeTitta and Joseph R. Luft and Angela M. Lauricella and Oleksandr Kalyuzhniy and Lori Anderson and Frank Zucker and Michael Soltis and Wim G. J. Hol},
  journal={Proteins: Structure},
The crystal structure of Pfal009167AAA, a putative ribulose 5‐phosphate 3‐epimerase (PfalRPE) from Plasmodium falciparum, has been determined to 2 Å resolution. RPE represents an exciting potential drug target for developing antimalarials because it is involved in the shikimate and the pentose phosphate pathways. The structure is a classic TIM‐barrel fold. A coordinated Zn ion and a bound sulfate ion in the active site of the enzyme allow for a greater understanding of the mechanism of action… Expand
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Eight key protein enzymes in the signal pathways were selected to perform molecular docking with artemisinin and it was suggested that purine nucleoside phosphorylase, peptide deformylase, and ribose 5-phosphate isomerase may be involved in the antimalarial mode of action of art Artemisinin. Expand
Crystal structure of D-psicose 3-epimerase from Agrobacterium tumefaciens and its complex with true substrate D-fructose: a pivotal role of metal in catalysis, an active site for the non-phosphorylated substrate, and its conformational changes.
Structural evidence and site-directed mutagenesis of the putative catalytic residues suggest that the metal ion plays a pivotal role in catalysis by anchoring the bound D-fructose, and Glu150 and GLU244 carry out an epimerization reaction at the C-3 position. Expand
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  • S. Ito
  • Chemistry, Medicine
  • Applied Microbiology and Biotechnology
  • 2009
This review surveys the catalytic aspects of microbial epimerases, which are relevant for production of bioactive mono- and oligosaccharides. Expand
CSGID Solves Structures and Identifies Phenotypes for Five Enzymes in Toxoplasma gondii
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Conversion of d‐ribulose 5‐phosphate to D‐xylulose 5‐phosphate: new insights from structural and biochemical studies on human RPE
It is shown that human D‐ribulose 5‐phosphate 3‐epimerase (hRPE) uses Fe2+ for catalysis, and the binary complexes reported here will aid in the design of small molecules for modulating the activity of the enzyme and altering flux through the PPP. Expand
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Structure of D-ribulose 5-phosphate 3-epimerase from Synechocystis to 1.6 A resolution.
The crystal structure of D-ribulose 5-phosphate 3-epimerase from the cyanobacterium Synechocystis was determined by X-ray crystallography to 1.6 A resolution and provides further evidence for essential catalytic roles for several active-site residues. Expand
Structure and catalytic mechanism of the cytosolic D-ribulose-5-phosphate 3-epimerase from rice.
Cytosolic D-ribulose-5-phosphate 3-epimerase from rice was crystallized after EDTA treatment and structurally elucidated by X-ray diffraction to 1.9A resolution. A prominent Zn(2+) site at the activeExpand
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Data mining of the transcriptome of Plasmodium falciparum: the pentose phosphate pathway and ancillary processes
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The goal of PlasmoDB is to facilitate utilization of the vast quantities of genomic-scale data produced by the global malaria research community. Expand
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The Plasmodium Genome Database provides the user with a variety of analysis tools for examining and extracting information from the genome and predicted proteome, using BLAST, electronic PCRs, defined motif searches, and tools for the analysis of microarray and proteomics data. Expand
Refinement of macromolecular structures by the maximum-likelihood method.
The likelihood function for macromolecular structures is extended to include prior phase information and experimental standard uncertainties and the results derived are consistently better than those obtained from least-squares refinement. Expand
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The ExPASy (the Expert Protein Analysis System) World Wide Web server (, is provided as a service to the life science community by a multidisciplinary team at the SwissExpand
The high-speed Hydra-Plus-One system for automated high-throughput protein crystallography.
  • H. Krupka, B. Rupp, +5 authors A. Azarani
  • Materials Science, Medicine
  • Acta crystallographica. Section D, Biological crystallography
  • 2002
The Hydra-Plus-One combines high precision, reliability and speed in a cost-effective high-throughput system ideally suited for protein crystallization. Expand