Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 A resolution.

@article{Longenecker2003StructureOA,
  title={Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 A resolution.},
  author={Kenton L Longenecker and Paul Read and Shin-Kai Lin and Andrew P. Somlyo and Robert K. Nakamoto and Zygmunt Derewenda},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={2003},
  volume={59 Pt 5},
  pages={876-80}
}
Mutants of the small G protein RhoA that are deficient in GTPase activity and thereby exhibit constitutive molecular signaling activity are commonly used to discover its cellular functions. In particular, two such mutants, Gly14-->Val (G14V) and Gln63-->Leu (Q63L), are often used interchangeably for such studies. However, while their in vitro rates of GTP hydrolysis are very similar, differences are observed in their other functional properties. The structure of G14V-RhoA is known; in order to… CONTINUE READING