Structure of Saccharomyces cerevisiae alg3, sec18 mutant oligosaccharides.

@article{Verostek1991StructureOS,
  title={Structure of Saccharomyces cerevisiae alg3, sec18 mutant oligosaccharides.},
  author={Mary Frances Verostek and Paul H Atkinson and Robert B Trimble},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 9},
  pages={5547-51}
}
Asparagine-linked oligosaccharides are synthesized by transfer of Glc3Man9GlcNAc2 from dolichol pyrophosphate to nascent polypeptides. Assembly of the precursor proceeds by highly ordered sequential addition of mannose and glucose to form Glc3Man9GlcNAc2-P-P-dolichol. Yeast mutants in asparagine-linked glycosylation (alg), generated by an 3H-Man suicide technique, were assigned to eight complementation groups which define steps in oligosaccharide-lipid synthesis (Huffaker, T.C., and Robbins, P… CONTINUE READING