Structure of Outer Hair Cell Stereocilia Side and Attachment Links in the Chinchilla Cochlea

@article{Tsuprun2002StructureOO,
  title={Structure of Outer Hair Cell Stereocilia Side and Attachment Links in the Chinchilla Cochlea},
  author={Vladimir Tsuprun and Peter Angelo Santi},
  journal={Journal of Histochemistry \& Cytochemistry},
  year={2002},
  volume={50},
  pages={493 - 502}
}
  • V. Tsuprun, P. Santi
  • Published 1 April 2002
  • Chemistry
  • Journal of Histochemistry & Cytochemistry
The structure and symmetry of chinchilla outer hair cell (OHC) stereocilia side and attachment links were investigated by transmission electron microscopy using tannic acid and Cuprolinic blue histochemical procedures. The side links run laterally between and across the rows of the stereocilia and connect the stereocilia together within the bundle. Attachment links form a crown-like array around the tips of only the tallest OHC stereocilia and attach these stereocilia to the Type B fibrils of… 

Figures from this paper

Structure of the stereocilia side links and morphology of auditory hair bundle in relation to noise exposure in the chinchilla
TLDR
Most disarrayed OHC and IHC stereocilia recovered to a normal erect state with restored orientation of the side links after 14–28 days, which correlated with near-complete recovery of auditory sensitivity, however, direct attachment of plasma membranes, ruptured links, fusion and blebs were seen on some stereOCilia even after 28 days and appear to be permanent.
Development and properties of stereociliary link types in hair cells of the mouse cochlea
TLDR
There is a rich, rapidly changing array of different links covering the developing hair bundle that becomes progressively refined to generate the mature complement by P19, as indicated by the results of electron microscopy.
Stereocilin connects outer hair cell stereocilia to one another and to the tectorial membrane
TLDR
It is proposed that horizontal top connectors, which maintain the cohesiveness of the mature OHC hair bundle, are required for tip‐link turnover in the mature cochlea.
The structure of tip links and kinocilial links in avian sensory hair bundles.
TLDR
The similar helix-like structure of the tip links and kinocilial links is in accord with the presence of a common cell-surface antigen (TLA antigen) and similarities in the physical and chemical properties of the two link types.
Otogelin, otogelin-like, and stereocilin form links connecting outer hair cell stereocilia to each other and the tectorial membrane
TLDR
It is suggested that these 2 OHC-specific structures have shared mechanical properties mediating reaction forces to sound-induced shearing motion and contributing to the coordinated displacement of stereocilia.
Analysis of the Dynamic Behavior of the Inner Hair Cell Stereocilia by the Finite Element Method
TLDR
It was suggested that the lateral links protect the MET channels located at taller stereocilia against large stimuli and subject the channels located in the same IHC to forces of similar size.
Hair-Bundle Links: Genetics as the Gateway to Function.
TLDR
Evidence showing CDH23 and PCDH15 are components of the tip, kinocilial, and transient-lateral links is reviewed, and insight is provided as to how the structure of tip link may contribute to the elastic element predicted to lie in series with the hair cell's mechanoelectrical transducer channel.
Dimensions of a Living Cochlear Hair Bundle
TLDR
The first complete blueprint of a living IHC hair bundle is created and it is shown that SEM-prepared measurements strongly affect calculations of a bundle’s mechanical properties – overestimating stereociliary deflection stiffness and underestimating the fluid coupling between stereocilia.
Sliding Adhesion Confers Coherent Motion to Hair Cell Stereocilia and Parallel Gating to Transduction Channels
TLDR
Observations suggest that horizontal top connectors mediate a sliding adhesion and indicate that all transduction channels of a hair cell are mechanically in parallel, an arrangement that may enhance amplification in the inner ear.
Structure and innervation of the cochlea
...
1
2
3
4
...

References

SHOWING 1-10 OF 46 REFERENCES
Structure of outer hair cell stereocilia links in the chinchilla
TLDR
The structure of side, tip, and “attachment” links of chinchilla outer hair cell (OHC) stereocilia was studied by transmission and scanning electron microscopy using tannic acid and Cuprolinic blue histochemical procedures and reactive structures appear to contain acidic, sulfated residues of proteoglycans or glycoproteins.
Helical Structure of Hair Cell Stereocilia Tip Links In the Chinchilla Cochlea
TLDR
Outer-hair-cell stereocilia tip-link structure in the chinchilla cochlea was studied by transmission electron microscopy using tannic acid and Ruthenium red/Alcian blue histochemical procedures to show that the strand is a three-start helical bundle of fine, coiled filaments which had an axial period of 22.5 ± 1.5 nm.
Stereociliary cross-links between adjacent inner hair cells
Surface charges of the membrane and cell adhesion substances determine the structural integrity of hair bundles from the inner ear of fish
TLDR
Transmission electron-microscopical investigation of sensory epithelia from two species of fish has shown that not only the connectors but also the surface charges of the membrane are important factors for determining the shape of the hair bundle and the spatial interrelation of the stereovilli.
Cross-links between stereocilia in the guinea pig cochlea
Immunoreactivity of sensory hair bundles of the guinea-pig cochlea to antibodies against elastin and keratan sulphate
TLDR
It is suggested that the stereociliary bundles of hair cells contain keratan sulphate proteoglycans, molecules which in other tissues are known to maintain structural integrity and fibrillar spacing, and to influence the microenvironment of the cell surface.
Morphology and cross-linkage of stereocilia in the guinea-pig labyrinth examined without the use of osmium as a fixative
TLDR
All the apical surface membranes of the hair cells appeared rough, and contained dense granules, and it is suggested that distortion of this link might be involved in the mechanics or even the membrane biophysics of sensory transduction.
...
1
2
3
4
5
...