Structure of NADP+-bound 7β-hydroxysteroid dehydrogenase reveals two cofactor-binding modes.

  title={Structure of NADP+-bound 7$\beta$-hydroxysteroid dehydrogenase reveals two cofactor-binding modes.},
  author={R. Wang and Jiaquan Wu and David Kin Jin and Yali Chen and Zhijia Lv and Q. Chen and Q. Miao and X. Huo and Feng Wang},
  journal={Acta crystallographica. Section F, Structural biology communications},
  volume={73 Pt 5},
  • R. Wang, Jiaquan Wu, +6 authors Feng Wang
  • Published 2017
  • Medicine, Biology
  • Acta crystallographica. Section F, Structural biology communications
In mammals, bile acids/salts and their glycine and taurine conjugates are effectively recycled through enterohepatic circulation. 7β-Hydroxysteroid dehydrogenases (7β-HSDHs; EC, including that from the intestinal microbe Collinsella aerofaciens, catalyse the NADPH-dependent reversible oxidation of secondary bile-acid products to avoid potential toxicity. Here, the first structure of NADP+ bound to dimeric 7β-HSDH is presented. In one active site, NADP+ adopts a conventional binding… Expand
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