Structure of Insoluble Rat Sperm Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) via Heterotetramer Formation with Escherichia coli GAPDH Reveals Target for Contraceptive Design*

@article{Frayne2009StructureOI,
  title={Structure of Insoluble Rat Sperm Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) via Heterotetramer Formation with Escherichia coli GAPDH Reveals Target for Contraceptive Design*},
  author={J. Frayne and A. Taylor and Gus Cameron and A. Hadfield},
  journal={The Journal of Biological Chemistry},
  year={2009},
  volume={284},
  pages={22703 - 22712}
}
  • J. Frayne, A. Taylor, +1 author A. Hadfield
  • Published 2009
  • Biology, Medicine
  • The Journal of Biological Chemistry
    • Sperm glyceraldehyde-3-phosphate dehydrogenase has been shown to be a successful target for a non-hormonal contraceptive approach, but the agents tested to date have had unacceptable side effects. Obtaining the structure of the sperm-specific isoform to allow rational inhibitor design has therefore been a goal for a number of years but has proved intractable because of the insoluble nature of both native and recombinant protein. We have obtained soluble recombinant sperm glyceraldehyde-3… CONTINUE READING
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