Structure of Insoluble Rat Sperm Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) via Heterotetramer Formation with Escherichia coli GAPDH Reveals Target for Contraceptive Design*

@article{Frayne2009StructureOI,
  title={Structure of Insoluble Rat Sperm Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) via Heterotetramer Formation with Escherichia coli GAPDH Reveals Target for Contraceptive Design*},
  author={J. Frayne and A. Taylor and Gus Cameron and A. Hadfield},
  journal={The Journal of Biological Chemistry},
  year={2009},
  volume={284},
  pages={22703 - 22712}
}
  • J. Frayne, A. Taylor, +1 author A. Hadfield
  • Published 2009
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • Sperm glyceraldehyde-3-phosphate dehydrogenase has been shown to be a successful target for a non-hormonal contraceptive approach, but the agents tested to date have had unacceptable side effects. Obtaining the structure of the sperm-specific isoform to allow rational inhibitor design has therefore been a goal for a number of years but has proved intractable because of the insoluble nature of both native and recombinant protein. We have obtained soluble recombinant sperm glyceraldehyde-3… CONTINUE READING
    37 Citations
    Structural analyses to identify selective inhibitors of glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme.
    • 14
    • Highly Influenced
    • PDF
    Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm
    • 21
    Sperm-Specific Glyceraldehyde-3-Phosphate Dehydrogenase–An Evolutionary Acquisition of Mammals
    • 6
    • Highly Influenced
    • PDF
    Partial catalytic Cys oxidation of human GAPDH.
    • Highly Influenced

    References

    SHOWING 1-10 OF 57 REFERENCES
    Structure of rabbit-muscle glyceraldehyde-3-phosphate dehydrogenase.
    • 72
    • PDF
    High-resolution structure of human D-glyceraldehyde-3-phosphate dehydrogenase.
    • J. Jenkins, J. Tanner
    • Biology, Medicine
    • Acta crystallographica. Section D, Biological crystallography
    • 2006
    • 94
    • PDF
    Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase.
    • 38
    • PDF
    Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility.
    • K. Miki, W. Qu, +6 authors D. O'brien
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 2004
    • 494
    • PDF