Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 Å Resolution

  title={Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 Å Resolution},
  author={Colin C. F. Blake and D. F. Koenig and Gerald Mair and A. C. North and David C. Phillips and V. R. Sarma},
Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 A Resolution 
Differences between the chemical structures of duck and hen egg-white lysozymes.
Histidine-free duck egg-white lysozyme III and hen egg-white lysozyme have similar but not identical structures.
Structural basis of antigen recognition: crystal structure of duck egg lysozyme
Lysozyme isoforms purified from duck eggs have been characterized by crystallography, mass spectrometry and their capacity to bind landmark antibodies.
The active site of hen egg-white lysozyme: flexibility and chemical bonding
Chemical bonding at the active site of lysozyme is analyzed on the basis of a multipole model employing transferable multipole parameters from a database. Large B factors at low temperatures reflect
Three-dimensional structure of thermolysin.
The electron density map of thermolysin indicates how the polypeptide chain is displaced throughout the overall shape of the molecule.
The lysozyme mechanism sorted — after 50 years
  • A. J. Kirby
  • Chemistry, Biology
    Nature Structural Biology
  • 2001
Unambiguous evidence for a glycosyl-enzyme intermediate on the lysozyme reaction pathway has recently been reported, finally settling what kind of mechanism this textbook enzyme uses.
Crystallization, structure solution and refinement of hen egg-white lysozyme at pH 8.0 in the presence of MPD.
Hen egg-white lysozyme has been crystallized at slightly alkaline pH using 2-methyl-2,4-pentanediol (MPD) as the precipitant. The crystals are nearly isomorphous to crystals grown at acidic pH using
Quantum Mechanics/Molecular Mechanics Simulations Show Saccharide Distortion is Required for Reaction in Hen Egg-White Lysozyme.
Hybrid quantum mechanics/molecular mechanics (QM/MM) calculations on lysozyme show significant distortion of the bound saccharide is required to facilitate the catalytic reaction.
Investigation of protein structure by means of 19F-NMR. A study of hen egg-white lysozyme.
A comparison of the circular dichroic spectra and the activity towards Micrococcus lysodeikticus of both LF6 and native lysozyme reveals that the labeling causes no major conformational changes of the polypeptide backbone.
Three-dimensional Fourier Synthesis of Tosyl-elastase at 3.5 Å Resolution
X-ray diffraction data have been collected from four isomorphous crystalline derivatives of the proteolytic enzyme elastase, and a map of the three-dimensional electron density of the tosyl


Structure Of Lysozyme: A Fourier Map of the Electron Density at 6 Å Resolution obtained by X-ray Diffraction
Structure Of Lysozyme: A Fourier Map of the Electron Density at 6 A Resolution obtained by X-ray Diffraction
The crystal structure of myoglobin: Phase determination to a resolution of 2 Å by the method of isomorphous replacement
The method of multiple isomorphous replacement has been used to solve the structure of the protein myoglobin to a resolution of 2 A. EDSAC II has been programmed to calculate the phase angles, and
Optical rotation and the conformation of polypeptides and proteins.
The theory of the method of isomorphous replacement for protein crystals. I
The average change of intensity due to adding a heavy atom to a protein crystal is calculated for both the centrie and the acentric reflections. The amount by which small shifts of the molecules
On a Remarkable Bacteriolytic Element Found in Tissues and Secretions
A substance present in the tissues and secretions of the body, which is capable of rapidly dissolving certain bacteria, is called a “Lysozyme,” and is alluded to as the Micrococcus lysodeikticus.
Adaptation of a linear diffractometer to measure three X-ray reflections quasi-simultaneously
A single-crystal linear diffractometer has been adapted to measure three reflections quasi-simultaneously in the flat-cone setting. Three counters are mounted in echelon, with their windows
The combination of isomorphous replacement and anomalous scattering data in phase determination of non-centrosymmetric reflexions
Earl ier a t t empts to combine anomalous scat ter ing da ta and isomorphous replacement da ta in phase de termina t ion are discussed. The relat ive weights given to the two types of observation
On the design of single crystal diffractometers to measure a number of reflections simultaneously
Single-crystal diffractometers derived from inclination Weissenberg goniometers can be adapted to measure more than one reflection at a time. Two reflections can be measured simultaneously, one from