Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 Å Resolution

@article{Blake1965StructureOH,
  title={Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 Å Resolution},
  author={C. Blake and D. F. Koenig and G. Mair and A. North and D. Phillips and V. R. Sarma},
  journal={Nature},
  year={1965},
  volume={206},
  pages={757-761}
}
Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 A Resolution 
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Structural insights into protein folding, stability and activity using in vivo perdeuteration of hen egg-white lysozyme
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The active site of hen egg-white lysozyme: flexibility and chemical bonding
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  • Physics, Medicine
  • Acta crystallographica. Section D, Biological crystallography
  • 2014
Chemical bonding at the active site of lysozyme is analyzed on the basis of a multipole model employing transferable multipole parameters from a database. Large B factors at low temperatures reflectExpand
The crystal structure of tortoise egg-white lysozyme at 6 A resolution.
TLDR
The crystals of tortoise egg-white lysozyme contain a large proportion of liquid and the X-ray map shows that this forms large channels through the crystals into which the active sites of the enzyme molecules open, indicating that tortois lyso enzyme crystals may be suitable for low-temperature studies of true enzyme substrate complexes. Expand
The lysozyme mechanism sorted — after 50 years
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  • Chemistry, Medicine
  • Nature Structural Biology
  • 2001
Unambiguous evidence for a glycosyl-enzyme intermediate on the lysozyme reaction pathway has recently been reported, finally settling what kind of mechanism this textbook enzyme uses.
Crystallization, structure solution and refinement of hen egg-white lysozyme at pH 8.0 in the presence of MPD.
Hen egg-white lysozyme has been crystallized at slightly alkaline pH using 2-methyl-2,4-pentanediol (MPD) as the precipitant. The crystals are nearly isomorphous to crystals grown at acidic pH usingExpand
Quantum Mechanics/Molecular Mechanics Simulations Show Saccharide Distortion is Required for Reaction in Hen Egg-White Lysozyme.
Hybrid quantum mechanics/molecular mechanics (QM/MM) calculations on lysozyme show significant distortion of the bound saccharide is required to facilitate the catalytic reaction.
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Structure Of Lysozyme: A Fourier Map of the Electron Density at 6 Å Resolution obtained by X-ray Diffraction
Structure Of Lysozyme: A Fourier Map of the Electron Density at 6 A Resolution obtained by X-ray Diffraction
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The crystal structure of myoglobin: Phase determination to a resolution of 2 Å by the method of isomorphous replacement
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The combination of isomorphous replacement and anomalous scattering data in phase determination of non-centrosymmetric reflexions
Earl ier a t t empts to combine anomalous scat ter ing da ta and isomorphous replacement da ta in phase de termina t ion are discussed. The relat ive weights given to the two types of observationExpand
On the design of single crystal diffractometers to measure a number of reflections simultaneously
Single-crystal diffractometers derived from inclination Weissenberg goniometers can be adapted to measure more than one reflection at a time. Two reflections can be measured simultaneously, one fromExpand
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