Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 Å Resolution

  title={Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 Å Resolution},
  author={C. Blake and D. F. Koenig and G. Mair and A. North and D. Phillips and V. R. Sarma},
Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 A Resolution 
Differences between the chemical structures of duck and hen egg-white lysozymes.
Histidine-free duck egg-white lysozyme III and hen egg-white lysozyme have similar but not identical structures.
X-ray analysis of structure of human lysozyme at 6 A resolution.
The tertiary structures of human and hen egg white lysozyme are very similar and a comparison of the two has provided some interesting results.
Structural basis of antigen recognition: crystal structure of duck egg lysozyme
Lysozyme isoforms purified from duck eggs have been characterized by crystallography, mass spectrometry and their capacity to bind landmark antibodies.
Structural insights into protein folding, stability and activity using in vivo perdeuteration of hen egg-white lysozyme
Perdeuteration and in vitro refolding of hen egg-white lysozyme impact protein thermal stability and activity through changes in protein dynamics, while refolding contributes to a small decrease inprotein thermal stability. Expand
Three-dimensional structure of thermolysin.
The electron density map of thermolysin indicates how the polypeptide chain is displaced throughout the overall shape of the molecule.
The active site of hen egg-white lysozyme: flexibility and chemical bonding
  • J. Held, S. van Smaalen
  • Physics, Medicine
  • Acta crystallographica. Section D, Biological crystallography
  • 2014
Chemical bonding at the active site of lysozyme is analyzed on the basis of a multipole model employing transferable multipole parameters from a database. Large B factors at low temperatures reflectExpand
The crystal structure of tortoise egg-white lysozyme at 6 A resolution.
The crystals of tortoise egg-white lysozyme contain a large proportion of liquid and the X-ray map shows that this forms large channels through the crystals into which the active sites of the enzyme molecules open, indicating that tortois lyso enzyme crystals may be suitable for low-temperature studies of true enzyme substrate complexes. Expand
The lysozyme mechanism sorted — after 50 years
  • A. J. Kirby
  • Chemistry, Medicine
  • Nature Structural Biology
  • 2001
Unambiguous evidence for a glycosyl-enzyme intermediate on the lysozyme reaction pathway has recently been reported, finally settling what kind of mechanism this textbook enzyme uses.
Crystallization, structure solution and refinement of hen egg-white lysozyme at pH 8.0 in the presence of MPD.
Hen egg-white lysozyme has been crystallized at slightly alkaline pH using 2-methyl-2,4-pentanediol (MPD) as the precipitant. The crystals are nearly isomorphous to crystals grown at acidic pH usingExpand
Quantum Mechanics/Molecular Mechanics Simulations Show Saccharide Distortion is Required for Reaction in Hen Egg-White Lysozyme.
Hybrid quantum mechanics/molecular mechanics (QM/MM) calculations on lysozyme show significant distortion of the bound saccharide is required to facilitate the catalytic reaction.


Structure Of Lysozyme: A Fourier Map of the Electron Density at 6 Å Resolution obtained by X-ray Diffraction
Structure Of Lysozyme: A Fourier Map of the Electron Density at 6 A Resolution obtained by X-ray Diffraction
Direct crystallization of lysozyme from egg white and some crystalline salts of lysozyme.
Crystallization was effected from acid solutions of purified material containing approximately 5 per cent salt, and it was also accomplished in the isoelectric region as a result of markedly lower solubility of the crystalline product in that region. Expand
The crystal structure of myoglobin: Phase determination to a resolution of 2 Å by the method of isomorphous replacement
The method of multiple isomorphous replacement has been used to solve the structure of the protein myoglobin to a resolution of 2 A. EDSAC II has been programmed to calculate the phase angles, andExpand
Optical rotation and the conformation of polypeptides and proteins.
An extension of the spectral range of measurement toward optically active absorption bands in the far ultraviolet is expected to yield new information about the rotatory power of the peptide bond and thus enhance the interaction of theory and observation that has already proved fruitful. Expand
The theory of the method of isomorphous replacement for protein crystals. I
The average change of intensity due to adding a heavy atom to a protein crystal is calculated for both the centrie and the acentric reflections. The amount by which small shifts of the moleculesExpand
On a Remarkable Bacteriolytic Element Found in Tissues and Secretions
A substance present in the tissues and secretions of the body, which is capable of rapidly dissolving certain bacteria, is called a “Lysozyme,” and is alluded to as the Micrococcus lysodeikticus. Expand
Adaptation of a linear diffractometer to measure three X-ray reflections quasi-simultaneously
A single-crystal linear diffractometer has been adapted to measure three reflections quasi-simultaneously in the flat-cone setting. Three counters are mounted in echelon, with their windowsExpand
The combination of isomorphous replacement and anomalous scattering data in phase determination of non-centrosymmetric reflexions
Earl ier a t t empts to combine anomalous scat ter ing da ta and isomorphous replacement da ta in phase de termina t ion are discussed. The relat ive weights given to the two types of observationExpand
On the design of single crystal diffractometers to measure a number of reflections simultaneously
Single-crystal diffractometers derived from inclination Weissenberg goniometers can be adapted to measure more than one reflection at a time. Two reflections can be measured simultaneously, one fromExpand