Structure of Gentlyase, the neutral metalloprotease of Paenibacillus polymyxa

@inproceedings{Ruf2013StructureOG,
  title={Structure of Gentlyase, the neutral metalloprotease of Paenibacillus polymyxa
},
  author={Armin Ruf and Martine Stihle and J. Benz and Manfred Schmidt and Harald Sobek},
  booktitle={Acta crystallographica. Section D, Biological crystallography},
  year={2013}
}
Gentlyase is a bacterial extracellular metalloprotease that is widely applied in cell culture and for tissue dissociation and that belongs to the family of thermolysin-like proteases. The structure of thermolysin has been known since 1972 and that of Bacillus cereus neutral protease since 1992. However, the structure determination of other Bacillus neutral proteases has been hindered by their tendency to cannibalistic autolysis. High calcium conditions that allow the concentration and… CONTINUE READING
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