Structure of Alcohol Oxidase from Pichia pastoris by Cryo-Electron Microscopy

  title={Structure of Alcohol Oxidase from Pichia pastoris by Cryo-Electron Microscopy},
  author={Janet Vonck and David N. Parcej and Deryck J Mills},
  booktitle={PloS one},
The first step in methanol metabolism in methylotrophic yeasts, the oxidation of methanol and higher alcohols with molecular oxygen to formaldehyde and hydrogen peroxide, is catalysed by alcohol oxidase (AOX), a 600-kDa homo-octamer containing eight FAD cofactors. When these yeasts are grown with methanol as the carbon source, AOX forms large crystalline arrays in peroxisomes. We determined the structure of AOX by cryo-electron microscopy at a resolution of 3.4 Å. All residues of the 662-amino… CONTINUE READING


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