Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases

  title={Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases},
  author={Zhengyang Li and L. Li and Yingyi Huo and Z. Chen and Yu Zhao and J. Huang and S. Jian and Z. Rong and Dingfeng Wu and J. Gan and Xiaojian Hu and J. Li and X. Xu},
  journal={Biotechnology for Biofuels},
  • Zhengyang Li, L. Li, +10 authors X. Xu
  • Published 2020
  • Chemistry, Medicine
  • Biotechnology for Biofuels
  • Background Esterases and lipases hydrolyze short-chain esters and long-chain triglycerides, respectively, and therefore play essential roles in the synthesis and decomposition of ester bonds in the pharmaceutical and food industries. Many SGNH family esterases share high similarity in sequences. However, they have distinct enzymatic activities toward the same substrates. Due to a lack of structural information, the detailed catalytic mechanisms of these esterases remain barely investigated… CONTINUE READING

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