Structure-function relationship of different domains of the rat corticotropin-releasing factor receptor.

@article{Sydow1997StructurefunctionRO,
  title={Structure-function relationship of different domains of the rat corticotropin-releasing factor receptor.},
  author={Sabine Sydow and Jelena Radulovic and Frank M. Dautzenberg and Joachim Spiess},
  journal={Brain research. Molecular brain research},
  year={1997},
  volume={52 2},
  pages={182-93}
}
The significance of different domains of corticotropin-releasing factor receptor, type 1, (CRFR1) for ligand binding and cAMP accumulation was investigated with C-terminally truncated forms of rat CRFR1 (rCRFR1) tagged by a sequence of six histidine residues (His-tag) to facilitate protein purification and identification. These different forms of the receptor were N-glycosylated and transported properly to the membranes of transfected mammalian cells as indicated by Western blot analysis and… CONTINUE READING