Structure function insights into elusive Mycobacterium tuberculosis protein Rv1916.

  title={Structure function insights into elusive Mycobacterium tuberculosis protein Rv1916.},
  author={Monika Antil and Jyoti Sharma and Yoan Brissonnet and Monika Choudhary and S{\'e}bastien G. Gouin and Vibha Gupta},
  journal={International journal of biological macromolecules},
Rv1915 and Rv1916 from Mycobacterium tuberculosis H37Rv form in vitro protein-protein complex.
Truncation of C-Terminal Intrinsically Disordered Region of Mycobacterial Rv1915 Facilitates Production of “Difficult-to-Purify” Recombinant Drug Target
This study reports optimization of solubilization buffer for recovery of soluble and biologically active recombinant mycobacterial Rv1915/ICL2a from IBs and truncated 90 residues from the C-terminal of Rv 1915/ ICL2 were truncated, supporting the disordered nature of R v19 15/ICl2a C- terminal.
Multi-Omics Approaches to Study Signaling Pathways


Mechanism-based inactivator of isocitrate lyases 1 and 2 from Mycobacterium tuberculosis
2-vinyl-d-isocitrate (2-VIC) is described as a mechanism-based inactivator of Mtb ICL1 and ICL2 that could provide a starting point for the development of new drugs to treat tuberculosis.
Active Site Flexibility of Mycobacterium tuberculosis Isocitrate Lyase in Dimer Form.
It is suggested that the open-closed behavior of the ICL active site entrance depends on the type of ligand present in the active site, and the elucidation of ICL dynamics can benefit future works such as lead identification or antibody design against ICL for TB therapeutics.
Isocitrate lyase mediates broad antibiotic tolerance in Mycobacterium tuberculosis.
Mycobacterium tuberculosis (Mtb) is a persistent intracellular pathogen intrinsically tolerant to most antibiotics. However, the specific factors that mediate this tolerance remain incompletely
Mycobacterium tuberculosis isocitrate lyases 1 and 2 are jointly required for in vivo growth and virulence
It is shown that prokaryotic and eukaryotic-like isoforms of the glyoxylate cycle enzyme isocitrate lyase (ICL) are jointly required for fatty acid catabolism and virulence in Mycobacterium tuberculosis.
Methylcitrate cycle defines the bactericidal essentiality of isocitrate lyase for survival of Mycobacterium tuberculosis on fatty acids
It is reported that Mtb’s ICLs are essential for survival on both acetate and propionate because of its methylisocitrate lyase (MCL) activity, and a previously unrecognized link between Propionate metabolism and membrane bioenergetics is revealed.
The role of RelMtb-mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice
  • J. L. Dahl, C. Kraus, C. Barry
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2003
RelMtb is critical for the successful establishment of persistent infection in mice by altering the expression of antigenic and enzymatic factors that may contribute to successful latent infection.
Menaquinone synthesis is critical for maintaining mycobacterial viability during exponential growth and recovery from non‐replicating persistence
In vitro assays indicate that the aminoalkoxydiphenylmethane derivatives specifically inhibit MenA, an enzyme involved in the synthesis of menaquinone, which provides insight into the physiology of mycobacterial persistence and a basis for the development of novel drugs that enhance eradication of persistent bacilli and latent tuberculosis.
Characterization of Activity and Expression of Isocitrate Lyase in Mycobacterium avium andMycobacterium tuberculosis
Express and purify Icl, as well as AceA proteins, and show that both exhibit isocitrate lyase activity, and it is presented evidence that in both M. avium and M. tuberculosis the production and activity of the isOCitrate Lyase is enhanced under minimal growth conditions when supplemented with acetate or palmitate.
Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS).
Methods described here, which have identified M. tuberculosis genes expressed in response to host interaction, will allow the study of gene expression in a variety of microorganisms, including expression resulting from interaction with human tissues in natural disease states.
The Three RelE Homologs of Mycobacterium tuberculosis Have Individual, Drug-Specific Effects on Bacterial Antibiotic Tolerance
The results suggest that, in vivo, RelE-generated persisters are unlikely to play a significant role in the generation of bacilli that survive in the face of multidrug therapy or in thegeneration of multodrug-resistant M. tuberculosis.