Structure-function assessment of the role of the helical stack domain in the properties of human recombinant protein C and activated protein C.

@article{Christiansen1995StructurefunctionAO,
  title={Structure-function assessment of the role of the helical stack domain in the properties of human recombinant protein C and activated protein C.},
  author={Wendy Christiansen and J P Geng and Francis J. Castellino},
  journal={Biochemistry},
  year={1995},
  volume={34 25},
  pages={8082-90}
}
The role of the helical stack (HS) in defining the properties of human recombinant (r) protein C (PC) and activated protein C (APC) was assessed. To do so, several mutations were made in this region of the molecule and their effects on the proteins examined. Substitution of the entire HS of PC (residues 38-46) by that of human coagulation factor (f) IX (residues 39-47), yielding r-[HSIX]PC, did not result in any substantial changes in the gamma-carboxyglutamic acid domain (GD)-related Ca(2… CONTINUE READING