Structure-function analysis of mutant RNA-dependent RNA polymerase complexes with VPg

  title={Structure-function analysis of mutant RNA-dependent RNA polymerase complexes with VPg},
  author={Chaojiang Gu and Tao Zeng and Yong Li and Zhenghui Xu and Zhongxi Mo and Congyi Zheng},
  journal={Biochemistry (Moscow)},
The replication of the foot-and-mouth disease virus (FMDV) genome is critically dependent upon the activity of a virally encoded RNA-dependent RNA polymerase (RdRp). In this study, four mutant RdRps of FMDV were isolated from viral quasi-species treated with ribavirin, of which two were single mutants (L123F and T381A) and two were double mutants (T291I/T381I and L123F/F244L). The mutant proteins were expressed in Escherichia coli and purified by His-bind resin chromatography. In combination… 

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