Structure determination of the disialylated poly-(N-acetyllactosamine)-containingO-linked carbohydrate chains of equine chorionic gonadotropin

@article{Hokke1994StructureDO,
  title={Structure determination of the disialylated poly-(N-acetyllactosamine)-containingO-linked carbohydrate chains of equine chorionic gonadotropin},
  author={Cornelis H Hokke and Marc J. H. Roosenboom and Jane E. Thomas-Oates and Johannis P. Kamerling and Johannes F. G. Vliegenthart},
  journal={Glycoconjugate Journal},
  year={1994},
  volume={11},
  pages={35-41}
}
The disialylated poly-(N-acetyllactosamine)-containingO-linked oligosaccharide alditols, released by alkaline borohydride treatment of the enzymicallyN-deglycosylated β-subunit of equine chorionic chonadotropin, were purified by fast protein liquid chromatography (FPLC) on Mono Q and analysed by fast ion bombardment mass spectrometry (FAB-MS) and1H-NMR spectroscopy. The identified oligosaccharide alditols have the following structure: $$\begin{gathered} Neu5Ac\alpha 2 - 3\left[ {Gal\beta 1… CONTINUE READING

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