Structure determination of a sulfated N-glycans, candidate for a precursor of the selectin ligand in bovine lung

@article{Murakami2006StructureDO,
  title={Structure determination of a sulfated N-glycans, candidate for a precursor of the selectin ligand in bovine lung},
  author={Tomonori Murakami and Shunji Natsuka and Shin-ichi Nakakita and Sumihiro Hase},
  journal={Glycoconjugate Journal},
  year={2006},
  volume={24},
  pages={195-206}
}
To clarify the structure of non-sialic acid anionic residue on N-glycans in the mammalian tissues, we have isolated sialidase-resistant anionic residue on N-glycans from bovine lung. Analyses by partial acid hydrolysis and glycosidase digestions combined with a two-dimensional HPLC mapping method revealed that the major sialidase-resistant anionic N-glycan had a fucosylbianntenary core structure. The anionic residue was identified as a sulfate ester by methanolysis, anion-exchange… CONTINUE READING