Structure-based design of an RNA-binding zinc finger.

@article{McColl1999StructurebasedDO,
  title={Structure-based design of an RNA-binding zinc finger.},
  author={Damian J McColl and C D Honchell and Alan D. Frankel},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1999},
  volume={96 17},
  pages={9521-6}
}
A structure-based approach was used to design RNA-binding zinc fingers that recognize the HIV-1 Rev response element (RRE). An arginine-rich alpha-helix from HIV-1 Rev was engineered into the zinc finger framework, and the designed fingers were shown to bind specifically to the RRE with high affinity and in a zinc-dependent manner, and display cobalt absorption and CD spectra characteristic of properly folded fingers. The results indicate that a monomeric zinc finger can recognize a specific… CONTINUE READING

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McColl et al

R. Tan, Frankel, 33 A.D.Biochemistry, 14579–14585. 9526 Biochemistry
Proc. Natl. Acad. Sci. USA 96 • 1999
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