Structure and stability of pertussis toxin studied by in situ atomic force microscopy.

@article{Yang1994StructureAS,
  title={Structure and stability of pertussis toxin studied by in situ atomic force microscopy.},
  author={Jae Hun Yang and Jingfang Mou and Zhengzhong Shao},
  journal={FEBS letters},
  year={1994},
  volume={338 1},
  pages={
          89-92
        }
}
Pertussis toxin, both complete and the B-oligomer, were imaged by atomic force microscopy (AFM), using specimens prepared by simple surface adsorption on mica without further manipulation. The spatial arrangement of the subunits of the B-oligomer was clearly resolved, representing the first protein quaternary structure obtained by AFM in situ. The results suggest that the B-oligomer is a flat pentamer with the two large subunits located next to each other, and the catalytic A-subunit situated… CONTINUE READING

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