Structure and orientation of sarcolipin in lipid environments.

@article{Mascioni2002StructureAO,
  title={Structure and orientation of sarcolipin in lipid environments.},
  author={Alessandro Mascioni and Christine B. Karim and George Barany and David D Thomas and Gianluigi Veglia},
  journal={Biochemistry},
  year={2002},
  volume={41 2},
  pages={
          475-82
        }
}
Sarcolipin (SLN) is a 31 amino acid integral membrane protein that regulates Ca-ATPase activity in skeletal muscle. Here, we report the three-dimensional structure and topology of synthetic SLN in lipid environments, as determined by solution and solid-state NMR spectroscopy. 2D solution NMR experiments were performed on SLN solubilized in sodium dodecyl sulfate (SDS) micelles. We found that SLN adopts a highly defined alpha-helical conformation from F9 through R27, with a backbone RMSD of 0.65… CONTINUE READING
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