Structure and gene cluster of the O-antigen of Enterobacter cloacae G3421.

@article{Perepelov2016StructureAG,
  title={Structure and gene cluster of the O-antigen of Enterobacter cloacae G3421.},
  author={Andrey V. Perepelov and Andrei V Filatov and Min Wang and A. S. J. H. U. Shashkov and Lei Wang and Yuriy A. Knirel},
  journal={Carbohydrate research},
  year={2016},
  volume={427},
  pages={55-9}
}
The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G3421 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. In addition, partial solvolysis with anhydrous trifluoroacetic acid was applied, which cleaved selectively the α-l-rhamnopyranosidic linkages. The following structure of the branched hexasaccharide repeating unit was established. The O-polysaccharide studied shares the β-l-Rhap-(1→4)-α-l-Rhap-(1→2… CONTINUE READING