Structure and functional relationships of archaeal and eukaryal histones and nucleosomes

  title={Structure and functional relationships of archaeal and eukaryal histones and nucleosomes},
  author={Kathleen Sandman and John N. Reeve},
  journal={Archives of Microbiology},
Abstract. A decade after the discovery of histones in Archaea, there is now also a biochemical description of the archaeal nucleosome. A tetrameric core of archaeal histones is encircled by ~80 bp of DNA, and nuclease digestions indicate that adjacent archaeal nucleosomes exist in vivo compacting archaeal genomic DNA. Most Eukarya employ a similar structure to organize their chromosomal DNA, the eukaryal nucleosome, with a histone octamer and 146 bp of DNA. Here we compare the properties of… 
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Both DNA and Histone Fold Sequences Contribute to Archaeal Nucleosome Stability*
If differences in this interface do result in archaeal histone cores with different sequence preferences, then the assembly of alternativearchaeal nucleosome tetramer cores could provide an unanticipated and novel structural mechanism to regulate gene expression.
Chromatin Polymorphism and the Nucleosome Superfamily: A Genealogy
Nucleosomes were discovered more than thirty years ago as the basic repeating units of chromatin and have progressively revealed their taste to come in many appearances, upon either adjunction of other proteins, histone substitution for isoforms (histone variants), depletion of one or the two H2A-H2B dimers (sub-nucleosome), intimate two-particle association, or isomeric structural alterations.
Nanoarchaeal Origin of Histone H3?
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An Archaeal Histone Is Required for Transformation of Thermococcus kodakarensis
The construction and properties of T. kodakarensis strains that have TK1413 or TK2289 deleted, the genes that encode HTkA and HTkB, respectively, the two archaeal histones present in this archaeon are reported.
Structure of Alba: an archaeal chromatin protein modulated by acetylation
A model for the Alba–DNA interaction is presented consistent with the available structural, biophysical and electron microscopy data.
Extended Archaeal Histone-Based Chromatin Structure Regulates Global Gene Expression in Thermococcus kodakarensis
Global transcriptome changes resultant from varying chromatin landscapes reveal the regulatory importance of higher-order histone-based chromatin architectures in regulating archaeal gene expression.
Physical and functional interaction of the archaeal single-stranded DNA-binding protein SSB with RNA polymerase.
The potential for promoter melting by SSB is demonstrated, suggesting a plausible basis for the stimulation of transcription and revealing an unexpected role for single-stranded DNA-binding proteins in transcription in archaea.
Archeal DNA replication: eukaryal proteins in a bacterial context.
In this review the current knowledge of the mechanisms governing DNA replication in archaea is summarized and the similarities and differences of those of bacteria and eukarya are highlighted.


Archaeal nucleosomes.
Results of experiments are reported that document that archaeal histones compact DNA in vivo into structures similar to the structure formed by the histone (H3+H4)2 tetramer at the center of the eukaryal nucleosome.
Diversity of prokaryotic chromosomal proteins and the origin of the nucleosome
The histone fold now exists not only in histones but also as a structural motif in eukaryal transcription factors, suggesting that histones may have prevailed during the evolution of the Eukarya.
Archaeal nucleosome positioning sequence from Methanothermus fervidus.
The translational and rotational positioning of archaeal nucleosome assembly in vitro by a sequence from the 7S RNA encoding region of the M. fervidus genome is documented.
Archaeal Nucleosome Positioning by CTG Repeats
It is documented that archaeal histones assemble archaealing nucleosomes in vitro centered preferentially within (CTG)6 and (CTg)8 repeats, close to junctions with flanking mixed-sequence DNA.
Identification and characterization of genomic nucleosome-positioning sequences.
This work isolated the DNA segments in the mouse genome that form the most stable nucleosomes yet characterized and selected sequences are shown to be localized at the centromeric regions of mouse metaphase chromosomes.
Histone stoichiometry and DNA circularization in archaeal nucleosomes.
Recombinant (r)HMfB (archaealhistone B fromMethanothermusfervidus) formed complexes with increasing stability with DNA molecules increasing in length from 52 to 100 bp, but not with a 39 bp molecule, and these complexes have been shown to contain an archaeal histone tetramer.
Contacts of the globular domain of histone H5 and core histones with DNA in a "chromatosome".
Evidence for an allosteric change in core histone-5S DNA interactions following the association of the linker histone in the nucleosome is discussed.
DNA binding and nuclease protection by the HMf histones from the hyperthermophilic archaeon Methanothermus fervidus
Abstract The DNA-binding and nuclease-protection properties of the HMf histones from the hyperthermophilic archaeon Methanothermus fervidus have been shown to be consistent with the formation of
DNA binding within the nucleosome core.