Structure and function of the photoreceptor stentorins in Stentor coeruleus. II. Primary photoprocess and picosecond time-resolved fluorescence.

@article{Song1990StructureAF,
  title={Structure and function of the photoreceptor stentorins in Stentor coeruleus. II. Primary photoprocess and picosecond time-resolved fluorescence.},
  author={Pill Soon Song and I. H. Kim and Scott R. Florell and Naoto Tamai and T. Yamazaki and Iwao Yamazaki},
  journal={Biochimica et biophysica acta},
  year={1990},
  volume={1040 1},
  pages={
          58-65
        }
}
Stentorin serves as the photoreceptor for the photophobic and negative phototactic responses in Stentor coeruleus. Two forms of the stentorin have been isolated and purified. The strongly fluorescent form, stentorin I at pH 7.8, exhibited nearly exponential fluorescence decay monitored at 620 nm, having two comparable lifetime decay components of 2.53 ns (47%) and 5.95 ns (53%). Stentorin I showed no significant time-resolved fluorescence emission spectra in the picosecond-nanosecond time… Expand
Initial spectroscopic characterization of the ciliate photoreceptor stentorin.
TLDR
Thepicosecond time-resolved fluorescence study revealed that the short picosecond emission component was the predominant emitting species in stentorin-2B and -2, followed by longer decaying species, and the possible mechanism for the primary photoreaction appears to involve electron transfer coupled with proton transfer. Expand
Structure and function of the photoreceptor stentorins in Stentor coeruleus. I. Partial characterization of the photoreceptor organelle and stentorins.
TLDR
Stentorin II appears to be the primary photoreceptor whose absorption and fluorescence properties are consistent with the action spectra for the photoresponses of the ciliate to visible light. Expand
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TLDR
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This analysis suggests that the excited state of the shorter wavelength-absorbing form of blepharismin undergoes primary photoprocess faster than that of the free parental chromophore hypericin. Expand
Electron Transfer Fluorescence Quenching of Blepharisma japonicum Photoreceptor Pigments
Abstract— The hypericin analogs blepharismin (BP), oxyblepharismin (OxyBP) and stentorin (ST), the photosensing chromophores responsible for photomotile reactions in the ciliates BlepharismaExpand
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TLDR
It is concluded that pigment granules of S. coeruleus function as organelles of defence against D. margaritifer and that the chemical basis of this defence is the pigment stentorin. Expand
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References

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Structure and function of the photoreceptor stentorins in Stentor coeruleus. I. Partial characterization of the photoreceptor organelle and stentorins.
TLDR
Stentorin II appears to be the primary photoreceptor whose absorption and fluorescence properties are consistent with the action spectra for the photoresponses of the ciliate to visible light. Expand
The pH dependence of photosensory responses in Stentor coeruleus and model system.
TLDR
The effect of external pH on net proton movement in the model system is strikingly similar to the pH dependent of the liver Stentor, thus lending support for transient proton flux being an important mode of light signal processing for photosensory transduction. Expand
Spectroscopic characterization of the Stentor photoreceptor.
TLDR
The photoreceptor chromophore released from mild acid hydrolysis has been identified as hypericin, and a mechanism for the photophobic response, based on this lowering of the pKa as the primary photoprocess, has been discussed. Expand
Deuterium oxide (D2O) enhances the photosensitivity of Stentor coeruleus.
TLDR
The results suggest that the sensory transduction mechanisms for the two photoresponses are different, although the photoreceptors (stentorin) are the same. Expand
Proton release from Stentor photoreceptors in the excited states.
Steady-state and picosecond pulse excitations of the photophobic-phototactic receptors isolated from Stentor coeruleus produced anionic species predominantly in the excited singlet state, althoughExpand
Primary photoprocesses of phytochrome. Picosecond fluorescence kinetics of oat and pea phytochromes.
TLDR
Comparison of the fluorescence decay data and the picosecond time-resolved fluorescence spectra suggests differences in conformational flexibility/heterogeneity among the preparations of the monocot vs dicot phy tochromes and the full-length native vs the amino terminus truncated phytochromes. Expand
EXCITATION ENERGY TRANSFER IN THE LIGHT HARVESTING ANTENNA SYSTEM OF THE RED ALGA Porphyridium cruentum AND THE BLUE‐GREEN ALGA Anacystis nidulans: ANALYSIS OF TIME‐RESOLVED FLUORESCENCE SPECTRA
TLDR
Overall time behaviors of the sequential fluorescence emissions from various pigments can be interpreted with a decay kinetics ofexp(–2kt½) and show that the energy transfer takes place much faster in the red alga P. cruentum than in the blue‐green alga A. nidulans. Expand
Picosecond studies of excited-state protonation and deprotonation kinetics. The laser pH jump
By measuring the time history of both anionic and acidic species under a variety of experimental conditions and using a kinetic scheme which includes terms for the fluorescence lifetimes andExpand
Microchannel‐plate photomultiplier applicability to the time‐correlated photon‐counting method
A microchannel‐plate photomultiplier (MCP‐PMT) has been applied to the time‐correlated single‐photon counting technique. Electrical and timing characteristics were investigated for the two types ofExpand
2‐Naphthol: A simple example of proton transfer effected by water structure
Using picosecond spectroscopic techniques, the proton transfer process of 2‐naphthol in its first excited singlet state is investigated in water/methanol mixtures at different temperatures. TheExpand
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