Structure and function of the photoreceptor stentorins in Stentor coeruleus. I. Partial characterization of the photoreceptor organelle and stentorins.

@article{Kim1990StructureAF,
  title={Structure and function of the photoreceptor stentorins in Stentor coeruleus. I. Partial characterization of the photoreceptor organelle and stentorins.},
  author={I. H. Kim and Jae-seong Rhee and Jae-wook Huh and Scott R. Florell and Brigitte Faure and K W Lee and T Z Kahsai and Pill Soon Song and Naoto Tamai and Tomoko Yamazaki},
  journal={Biochimica et biophysica acta},
  year={1990},
  volume={1040 1},
  pages={
          43-57
        }
}
The unicellular ciliary protozoan, Stentor coeruleus, exhibits photophobic and phototactic responses to visible light stimuli. The pigment granule contains the photoreceptor chromoproteins (stentorins). Stentorin localized in the pigment granules of the cell serves as the primary photoreceptor for the photophobic and phototactic responses in this organism. An initial characterization of the pigment granules has been described in terms of size, absorbance spectra and ATPase activity. Two forms… Expand
Initial spectroscopic characterization of the ciliate photoreceptor stentorin.
TLDR
Thepicosecond time-resolved fluorescence study revealed that the short picosecond emission component was the predominant emitting species in stentorin-2B and -2, followed by longer decaying species, and the possible mechanism for the primary photoreaction appears to involve electron transfer coupled with proton transfer. Expand
Structure and function of the photoreceptor stentorins in Stentor coeruleus. II. Primary photoprocess and picosecond time-resolved fluorescence.
TLDR
Results are suggestive of proton dissociation as a primary photoprocess from the excited state of stentorin II, and the fluorescence emission spectrum was time-resolvable in the picosecond time scales. Expand
Defence function of pigment granules in Stentor coeruleus
TLDR
It is concluded that pigment granules of S. coeruleus function as organelles of defence against D. margaritifer and that the chemical basis of this defence is the pigment stentorin. Expand
Chapter 17 Photomovement in ciliates
TLDR
Experimental lines of evidence indicated that photobehavioral responses in these ciliates are modulated by protonophores, ammonium chloride and extracellular pH, and it is hypothesized that a proton translocation occurs in the photoreceptor apparatus. Expand
Photosensory transduction in unicellular eukaryotes: a comparison between related ciliates Blepharisma japonicum and Stentor coeruleus and photoreceptor cells of higher organisms.
TLDR
Data indicate that both ciliates Blepharisma japonicum and Stentor coeruleus, the lower eukaryotes, are capable of transducing the perceived light stimuli in a manner taking place in some photoreceptor cells of higher eukARYotes. Expand
SPECTROSCOPIC AND PHOTOACOUSTIC STUDIES OF HYPERICIN EMBEDDED IN LIPOSOMES AS A PHOTORECEPTOR MODEL*
TLDR
From the analysis of fluorescence lifetimes and their relative amplitudes it appears that a subnanosecond living component is predominant at the highest hypericin local concentrations. Expand
Multifunctions of photodynamic pigments in ciliated protozoans
Quinone derivatives contained in colored heterotrichous ciliates such as Stentor coeruleus and the species belonging to the genus Blepharisma play multifunctional roles (by engaging in antimicrobialExpand
PRESUMED PHOTORECEPTOR PROTEIN AND ULTRASTRUCI'URE OF THE PHOTORECEPTOR ORGANELLE IN THE CILIATED PROTOZOAN, Blepharisma
Abstract‐The red pigment granule of Belpharisma japonicum is believed to be a photoreceptor organelle mediating photodispersal. Freeze‐fracture and thin section electron microscopy revealed that theExpand
Photophysics and Multifunctionality of Hypericin‐Like Pigments in Heterotrich Ciliates: A Phylogenetic Perspective
TLDR
The literature is reviewed and some new data is presented to examine the occurrence and photophysics of the diverse hypericin‐like chromophores in heterotrichs, the photoresponses of the cells, the various roles of the pigments and the taxa that might be studied to advance the understanding of these pigments. Expand
Time-resolved fluorescence spectroscopy and photolysis of the photoreceptor blepharismin.
TLDR
This analysis suggests that the excited state of the shorter wavelength-absorbing form of blepharismin undergoes primary photoprocess faster than that of the free parental chromophore hypericin. Expand
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Structure and function of the photoreceptor stentorins in Stentor coeruleus. II. Primary photoprocess and picosecond time-resolved fluorescence.
TLDR
Results are suggestive of proton dissociation as a primary photoprocess from the excited state of stentorin II, and the fluorescence emission spectrum was time-resolvable in the picosecond time scales. Expand
Spectroscopic characterization of the Stentor photoreceptor.
TLDR
The photoreceptor chromophore released from mild acid hydrolysis has been identified as hypericin, and a mechanism for the photophobic response, based on this lowering of the pKa as the primary photoprocess, has been discussed. Expand
Phototaxis and photophobic responses in Stentor coeruleus. Action spectrum and role of Ca2+ fluxes.
TLDR
A change in the response time decreases the degree of phototactic orientation, indicating that negative phototaxis in this organism is brought about by subsequent phobic responses of individual rows of cilia as the associated photoreceptor granules experience an increase in light intensity when the organism rotates during forward locomotion in lateral light. Expand
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TLDR
Evidence is presented that phototactic orientation is brought about by repetitive photophobic responses, since a change in the phobic response time results in a decreased accuracy of phototaxis. Expand
CHANGES IN cAMP AND cGMP CONCENTRATION, BIREFRINGENT FIBRILS AND CONTRACTILE ACTIVITY ACCOMPANYING UV AND BLUE LIGHT PHOTOAVOIDANCE IN PLASMODIA OF AN ALBINO STRAIN OF Physarum polycephalum
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TLDR
The photoreceptor systems of the ciliates Stentor and Blepharisma, the flagellate Euglena, and the filamentous alga Mougeotia are described and selected photosynthetic algae are discussed briefly. Expand
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PHOTOTACTIC ORIENTATION BY THE CILIATE, STENTOR COERULEUS
TLDR
The negative phototaxis was established by demonstrating the swimming of Stentor toward a focused beam away from the light source, and the action spectrum showed a maximum at 610–620 nm and is essentially identical to that of the step‐up photophobic response. Expand
Identification and characterization of a nuclear pore complex protein
TLDR
Using a monoclonal antibody that recognizes a 62 kd protein (p62) of rat liver nuclei, p62 is synthesized as a soluble cytoplasmic precursor of 61 kd, which is incorporated into the nuclear fraction with an unusually long t1/2 of about 6 hr. Expand
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Abstract Mutants of T4 which contain a duplication of the rII region also often contain a compensating deletion of a nonessential segment of the genome. This relationship was used to isolate a seriesExpand
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