Structure and function of the Zn(II) binding site within the DNA-binding domain of the GAL4 transcription factor.

@article{Pan1989StructureAF,
  title={Structure and function of the Zn(II) binding site within the DNA-binding domain of the GAL4 transcription factor.},
  author={Tao Pan and Joseph E. Coleman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1989},
  volume={86 9},
  pages={3145-9}
}
The transcription factor GAL4 from Saccharomyces cerevisiae contains a "zinc-finger"-like motif, Cys-Xaa2-Cys-Xaa6-Cys-Xaa6-Cys-Xaa2-Cys-Xaa6+ ++-Cys, within its DNA-binding domain. A GAL4 fragment consisting of residues 1-147 plus two additional residues from the cloning vector [denoted GAL4(149*)] has been cloned and overexpressed in Escherichia coli. This fragment includes the entire DNA-binding domain (residues 1-74). The homogeneous GAL4-(149*) protein contains 1-1.5 moles of Zn(II) per… CONTINUE READING

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