Structure and function in rhodopsin: expression of functional mammalian opsin in Saccharomyces cerevisiae.

@article{Mollaaghababa1996StructureAF,
  title={Structure and function in rhodopsin: expression of functional mammalian opsin in Saccharomyces cerevisiae.},
  author={Ramin Mollaaghababa and Florence F. Davidson and Constanze Kaiser and Har Gobind Khorana},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 21},
  pages={11482-6}
}
The yeast Saccharomyces cerevisiae has been investigated for expression of mammalian opsin as an alternative to the currently used expression in COS-1 mammalian cells. The synthetic opsin gene was placed under the control of the inducible promoter GAL1 in the multicopy yeast/ Escherichia coli shuttle vector YEpRF1. Transformation of a GAL+ S. cerevisiae strain with the vector and growth of galactose-induced cultures to saturation showed the production of 2.0 +/- 0.5 mg of opsin from about 10(10… CONTINUE READING