Structure and expression of the vasoactive intestinal peptide (VIP) gene in a human tumor

  title={Structure and expression of the vasoactive intestinal peptide (VIP) gene in a human tumor},
  author={Illana Gozes and Mordechai Bodner and Yael Shani and Mati Fridkin},

Vasoactive Intestinal Peptide Gene: Putative Mechanism of Information Storage at the RNA Level

It is demonstrated that an intron‐containing precursor RNA can be the major vasoactive intestinal peptide‐related RNA in the cell, which is in contrast to most known genes.

VIP: Molecular biology and neurobiological function

VIP is a major factor in brain activity, neuroendocrine functions, cardiac activity, respiration, digestion, and sexual potency, and exerts its function via receptor-mediated systems, activating signal transduction pathways, including cAMP.

Sequential expression in the nervous system of C-MYB andVIP genes, located in human chromosomal region 6q24

The protooncogene c-myb may be associated with events in brain development occurring prior to the appearance of elevated concentrations of VIP, and the possible correlation of c- mybto VIPin neuronal tissue is investigated.

Vasoactive Intestinal Peptide: From Molecular Genetics to Neurotropism

Inervation controls neuropeptide expression, and lesions of the optic nerve during development resulted in changes in brain VIP mRNA, and day-night cycles cause rhythmical alteration in the levels of VIP mRNA.

The gene encoding vasoactive intestinal peptide is located on human chromosome 6p21→6qter

It is concluded that the VIP-gene is not the primary gene defect in cystic fibrosis, as the CF gene was recently mapped to chromosome 7.

VIP, From Gene to Behavior and Back: Summarizing my 25 Years of Research

  • I. Gozes
  • Biology
    Journal of Molecular Neuroscience
  • 2008
This short review summarizes my own point of view and encompasses 25 years of work and over 100 publications targeting the understanding of VIP production and biological activity to identify novel VIP analogs that helped decipher VIP’s important role during development, in regulation of the biological clock(s) and diurnal rhythms, sexual activity, learning and memory as well as social behavior, and cancer.

Learning and sexual deficiencies in transgenic mice carrying a chimeric vasoactive intestinal peptide gene

To directly assess genetically reduced VIP content as a cause for learning impairment, transgenic mice carrying diphtheria toxia-encoding sequences driven by the rat VIP promoter were created, strongly supporting an important neurobiological function for VIP in vivo.



Coding sequences for vasoactive intestinal peptide and PHM-27 peptide are located on two adjacent exons in the human genome.

The occurrence of VIP and PHM-27 coding sequences on two separate exons of the human genome and the homology of their 3' splice site may allow alternative RNA processing as discussed below.

Detection of mRNAs containing regulatory peptide coding sequences using synthetic oligodeoxynucleotides

This work identified and partially purified a ∼ 1600‐base long mRNA containing VIP related sequences which can be translated in vitro into VIP‐immunoreactive polypeptides and differs in size from a known VIP‐mRNA identified in human neuro‐blastoma cells, suggesting the possibility of different VIP‐ mRNAs in different cell types.

Human preprovasoactive intestinal polypeptide contains a novel PHI-27-like peptide, PHM-27

The entire amino acid sequence of the precursor, deduced from the nucleotide sequence, indicates that the precursor protein contains not only VIP but also a novel peptide of 27 amino acids.

Exon duplication and divergence in the human preproglucagon gene

The data suggest that triplication and subsequent sequence divergence of an exon encoding glucagon or a glucagon-like peptide produced this polyprotein precursor.

Glucagon gene sequence. Four of six exons encode separate functional domains of rat pre-proglucagon.

The structure of the gene encoding rat pre-proglucagon, a polyprotein precursor of glucagon, is reported, which consists of six exons and five introns and contains repetitive sequence DNA.

Gene encoding human growth hormone-releasing factor precursor: structure, sequence, and chromosomal assignment.

Dot-blot analysis of DNA from high resolution dual-laser-sorted human chromosomes indicates that the single-copy growth hormone-releasing factor gene is located on human chromosome 20.

The distribution and chromatographic characterization of PHI (peptide histidine isoleucine amide)-27-like peptides in rat and porcine brain

Using specific PHI and VIP radioimmunoassays, the distribution of PLP was found to parallel that of VIP in rat and porcine brain, being highest in cerebral cortex, amygdala, and hippocampus.