Structure and expression of elongation factor Tu from Bacillus stearothermophilus.

  title={Structure and expression of elongation factor Tu from Bacillus stearothermophilus.},
  author={Libor Kr{\'a}sn{\'y} and Jeroen R. Mesters and L N Tieleman and Barend Kraal and Vladim{\'i}r Fu{\vc}{\'i}k and Rolf Hilgenfeld and Jiř{\'i} Jon{\'a}k},
  journal={Journal of molecular biology},
  volume={283 2},
The tuf gene coding for elongation factor Tu (EF-Tu) of Bacillus stearothermophilus was cloned and sequenced. This gene maps in the same context as the tufA gene of Escherichia coli str operon. Northern-blot analysis and primer extension experiments revealed that the transcription of the tuf gene is driven from two promoter regions. One of these is responsible for producing a 4.9-kb transcript containing all the genes of B. stearothermophilus str operon and the other, identified adjacent to the… CONTINUE READING


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