Structure and expression of an amylopullulanase gene from Bacillus stearothermophilus TS-23.

@article{Chen2001StructureAE,
  title={Structure and expression of an amylopullulanase gene from Bacillus stearothermophilus TS-23.},
  author={J T Chen and M. C. Chen and Lei Chen and Wei Sing Chu},
  journal={Biotechnology and applied biochemistry},
  year={2001},
  volume={33 Pt 3},
  pages={189-99}
}
An amylopullulanase gene (apuTS) from Bacillus stearothermophilus TS-23 was cloned and characterized. apuTS consisted of an open reading frame of 6054 bp encoding a protein of 2018 amino acids with a calculated M(r) of 223811. The deduced amino acid sequence revealed four highly conserved regions that are common among amylolytic enzymes. In the C-terminal region, a six-amino-acid sequence (Pro-Gly-Ser-Gly-Thr-Thr) is repeated nine times. It shared the highest degree of homology with the… CONTINUE READING
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