Structure and expression inE. coli of the gene coding for protein p10 of African swine fever virus

@article{Muoz2005StructureAE,
  title={Structure and expression inE. coli of the gene coding for protein p10 of African swine fever virus},
  author={Miguel Mu{\~n}oz and Jos{\'e} M P Freije and Mar{\'i}a L. Salas and E. Vinuela and Carlos L{\'o}pez-Ot{\'i}n},
  journal={Archives of Virology},
  year={2005},
  volume={130},
  pages={93-107}
}
The gene encoding protein p10, a structural protein of African swine fever (ASF) virus, has been mapped, sequenced and expressed inE. coli. Protein p10 was purified from dissociated virus by reverse-phase HPLC, and its NH2-terminal end identified by automated Edman degradation. To map the gene encoding protein p10, a mixture of 20-mer oligonucleotides based upon a part of the amino acid sequence was hybridized to cloned ASF virus restriction fragments. This allowed the localization of the gene… CONTINUE READING
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