Structure and dynamics of the Golgi complex at 15 degrees C: low temperature induces the formation of Golgi-derived tubules.

@article{MartnezAlonso2005StructureAD,
  title={Structure and dynamics of the Golgi complex at 15 degrees C: low temperature induces the formation of Golgi-derived tubules.},
  author={Emma Mart{\'i}nez-Alonso and Gustavo Egea and Jos{\'e} Ballesta and Jos{\'e} A Mart{\'i}nez-Men{\'a}rguez},
  journal={Traffic},
  year={2005},
  volume={6 1},
  pages={32-44}
}
Immunofluorescence and cryoimmunoelectron microscopy were used to examine the morphologic and functional effects on the Golgi complex when protein transport is blocked at the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) in HeLa cells incubated at low temperature (15 degrees C). At this temperature, the Golgi complex showed long tubules containing resident glycosylation enzymes but not matrix proteins. These Golgi-derived tubules also lacked anterograde (VSV-G) or retrograde… CONTINUE READING

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