Structure and dynamics in solution of the complex of lactobacillus casei dihydrofolate reductase with the new lipophilic antifolate drug trimetrexate

@article{Polshakov1999StructureAD,
  title={Structure and dynamics in solution of the complex of lactobacillus casei dihydrofolate reductase with the new lipophilic antifolate drug trimetrexate},
  author={Vladimir I. Polshakov and Berry Birdsall and Thomas A. Frenkiel and Angelo R. Gargaro and James Feeney},
  journal={Protein Science},
  year={1999},
  volume={8}
}
We have determined the three‐dimensional solution structure of the complex of Lactobacillus casei dihydrofolate reductase and the anticancer drug trimetrexate. Two thousand seventy distance, 345 dihedral angle, and 144 hydrogen bond restraints were obtained from analysis of multidimensional NMR spectra recorded for complexes containing 15N‐labeled protein. Simulated annealing calculations produced a family of 22 structures fully consistent with the constraints. Several intermolecular protein… 
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TLDR
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TLDR
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