Structure and composition of synaptonemal complexes, isolated from rat spermatocytes.

  title={Structure and composition of synaptonemal complexes, isolated from rat spermatocytes.},
  author={Christa Heyting and Axel J. J. Dietrich and Egbert J. W. Redeker and Agnes C. G. Vink},
  journal={European journal of cell biology},
  volume={36 2},
Synaptonemal complexes (SCs) (structures involved in chromosome pairing during meiosis) were isolated and purified from rat spermatocytes for the purpose of biochemical and morphological analysis. Spermatocytes were lysed in a medium, containing Triton X-100, EDTA and DTT; the resulting swollen nuclei were disrupted by DNAse II, and the suspension was centrifuged through 1.5 M sucrose. The resulting preparation consisted for at least 60% of free SCs, as judged from electron micrographs of agar… CONTINUE READING


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