The structure and cell composition of spheres obtained by culturing human fetal retinal cells after 15, 18, 22–23, and 24 weeks of gestation were studied. The cells were cultured as neurospheres: in serum-free medium with growth factors, in which they formed floating spheres. Immunocytochemical analysis showed that cell proliferation in the spheres decreased with increasing fetal age. Stem/progenitor cells, neuroblasts, and photoreceptors were detected in the spheres. Glial cells were detected only in spheres originating from 22-and 24-week fetuses. All spheres, irrespective of age and duration of culturing, consisted of numerous cell rosettes, each histotypically similar to the neuroblastic layer of the developing retina.