Structure and catalytic inactivity of the bacterial luciferase neutral flavin radical.

@article{Kurfrst1982StructureAC,
  title={Structure and catalytic inactivity of the bacterial luciferase neutral flavin radical.},
  author={M Kurf{\"u}rst and Sandro Ghisla and R P Presswood and John Woodland Hastings},
  journal={European journal of biochemistry},
  year={1982},
  volume={123 2},
  pages={355-61}
}
A luciferase-bound neutral flavin semiquinone radical can be formed upon the oxidation of the luciferase-FMNH2 complex by molecular oxygen. This species can also be formed anaerobically by comproportionation of FMN and FMNH2 in the presence of luciferase. The radical is kinetically stable (t1/2 approximately 20 h at 0 degree C in air; the Arrhenius delta H not equal to decay being about 170 kJ/mol) and can be prepared in pure form by Sephadex G-25 chromatography at 0-4 degrees C. The pure… CONTINUE READING