Structure and activity of the nitrate-reducing community in the rhizosphere of Lolium perenne and Trifolium repens under long-term elevated atmospheric pCO.

@article{Deiglmayr2004StructureAA,
  title={Structure and activity of the nitrate-reducing community in the rhizosphere of Lolium perenne and Trifolium repens under long-term elevated atmospheric pCO.},
  author={Kathrin Deiglmayr and Laurent Philippot and Ueli A. Hartwig and Ellen Kandeler},
  journal={FEMS microbiology ecology},
  year={2004},
  volume={49 3},
  pages={445-54}
}
Rhizosphere soil was sampled in monocultures of Lolium perenne and Trifolium repens in June and October 2002, at two different nitrogen fertilisation levels (14 and 56 g N m(-2) year(-1)) and under two pCO(2) atmospheres (360 and 600 ppmv) at the Swiss FACE (Free Air Carbon dioxide Enrichment) site. Directly extracted soil DNA was analysed with restriction fragment length polymorphism (PCR-RFLP) by use of degenerated primers for the narG gene encoding the active site of the membrane-bound… CONTINUE READING