Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica.

  title={Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica.},
  author={S. Lemak and A. Tchigvintsev and Pierre Petit and Robert L Flick and A. Singer and G. Brown and E. Evdokimova and O. Egorova and C. Gonzalez and T. Chernikova and M. Yakimov and M. Kube and R. Reinhardt and P. Golyshin and A. Savchenko and A. Yakunin},
  journal={The Biochemical journal},
  volume={445 2},
  • S. Lemak, A. Tchigvintsev, +13 authors A. Yakunin
  • Published 2012
  • Chemistry, Medicine
  • The Biochemical journal
  • The uncharacterized α/β-hydrolase protein OLEI01171 from the psychrophilic marine bacterium Oleispira antarctica belongs to the PF00756 family of putative esterases, which also includes human esterase D. In the present paper we show that purified recombinant OLEI01171 exhibits high esterase activity against the model esterase substrate α-naphthyl acetate at 5-30°C with maximal activity at 15-20°C. The esterase activity of OLEI01171 was stimulated 3-8-fold by the addition of chloride or several… CONTINUE READING
    21 Citations
    Structural and functional characterization of a novel cold-active S-formylglutathione hydrolase (SfSFGH) homolog from Shewanella frigidimarina, a psychrophilic bacterium
    • 3
    • Highly Influenced
    Structural and functional analysis of a low-temperature-active alkaline esterase from South China Sea marine sediment microbial metagenomic library
    • Y. Hu, Y. Liu, +4 authors Song Xue
    • Biology, Medicine
    • Journal of Industrial Microbiology & Biotechnology
    • 2015
    • 15
    The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes
    • 48
    • PDF
    A cold-adapted, solvent and salt tolerant esterase from marine bacterium Psychrobacter pacificensis.
    • 27
    Biochemical and Structural Characterization of a Five-domain GH115 α-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T*
    • 8
    • PDF
    Screening and Characterization of Novel Polyesterases from Environmental Metagenomes with High Hydrolytic Activity against Synthetic Polyesters.
    • 10
    • PDF


    Covalent reaction intermediate revealed in crystal structure of the Geobacillus stearothermophilus carboxylesterase Est30.
    • Ping Liu, Y. Wang, +4 authors I. Weber
    • Chemistry, Medicine
    • Journal of molecular biology
    • 2004
    • 54
    The crystal structure of Pyrococcus furiosus ornithine carbamoyltransferase reveals a key role for oligomerization in enzyme stability at extremely high temperatures.
    • 123
    • PDF
    Molecular Basis of Formaldehyde Detoxification
    • 77
    • PDF
    Structural basis of α‐amylase activation by chloride
    • 67
    • PDF