Structure and activity of the Saccharomyces cerevisiae dUTP pyrophosphatase DUT1, an essential housekeeping enzyme.

@article{Tchigvintsev2011StructureAA,
  title={Structure and activity of the Saccharomyces cerevisiae dUTP pyrophosphatase DUT1, an essential housekeeping enzyme.},
  author={A. Tchigvintsev and A. Singer and Robert L Flick and Pierre Petit and G. Brown and E. Evdokimova and A. Savchenko and A. Yakunin},
  journal={The Biochemical journal},
  year={2011},
  volume={437 2},
  pages={
          243-53
        }
}
  • A. Tchigvintsev, A. Singer, +5 authors A. Yakunin
  • Published 2011
  • Biology, Medicine
  • The Biochemical journal
  • Genomes of all free-living organisms encode the enzyme dUTPase (dUTP pyrophosphatase), which plays a key role in preventing uracil incorporation into DNA. In the present paper, we describe the biochemical and structural characterization of DUT1 (Saccharomyces cerevisiae dUTPase). The hydrolysis of dUTP by DUT1 was strictly dependent on a bivalent metal cation with significant activity observed in the presence of Mg2+, Co2+, Mn2+, Ni2+ or Zn2+. In addition, DUT1 showed a significant activity… CONTINUE READING
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